QUESTION 24 Answer Assume that a given plasmid vector to be used in a cloning experiment contains 4000 base pairs of DNA. Assume also that the restriction endonuclease Cul cuts this plasmid at the following sites (starting from an arbitrary zero point): 1000, 1500, and 3000 Given complete digestion of the plasmid with the endonuclease so that only linear fragments are produced, what sizes of DNA are expected? (Hint: drow the picture of plasmid and mark restriction points on it)

QUESTION 24 Answer Assume that a given plasmid vector to be used in a cloning experiment contains 4000 base pairs of DNA. Assume also that the restriction endonuclease Cul cuts this plasmid at the following sites (starting from an arbitrary zero point): 1000, 1500, and 3000 Given complete digestion of the plasmid with the endonuclease so that only linear fragments are produced, what sizes of DNA are expected? (Hint: drow the picture of plasmid and mark restriction points on it)

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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