1 2 3 kD 97 66 43 20 -D 14 In the protein gel above, Lane 1 shows the protein profile of E. coli BL21(DE3) cells containing an empty expression vector. Lanes 2 and 3 show protein profiles of E. coli BL21(DE3) cells that have been transformed with an expression vector containing an ORF encoding a protein of interest. Which band(s) could represent(s) the expression of heterologous protein?
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
I believe the answer would be A and D, but I am not sure because the recombinant proteins are significant only in the last to lanes. As the first lane is an empty vector. I appreciate your help.
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