**Educational Text Transcription:**5) PCR primers have a melting temperature (Tm) at which they become dissociated from the template DNA. This Tm is calculated by their length and their base composition. Suppose you have two PCR primers that are the exactly same length (each 20 nucleotides long). Primer A has a Tm of 60°C and Primer B has a Tm of 63°C. Based on what you know about DNA structure, hypothesize why there is a difference in Tm between the two primers?**Explanation:**The question refers to the concept of melting temperature (Tm) for PCR primers. The Tm is a critical parameter that affects how primers bind to DNA during the polymerase chain reaction. Although both primers are the same length, their differing Tm values suggest differences in base composition. Typically, a higher Tm indicates a higher concentration of guanine (G) and cytosine (C) bases, as G-C base pairs have three hydrogen bonds compared to the two hydrogen bonds of adenine (A) and thymine (T) pairs. Therefore, Primer B, with a higher Tm, likely has a higher G-C content than Primer A.

Name: **Educational Text Transcription:**5) PCR primers have a melting temp
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: **Educational Text Transcription:**5) PCR primers have a melting temperature (Tm) at which they become dissociated from the template DNA. This Tm is calculated by their length and their base composition. Suppose you have two PCR primers that are the