Answered: Why is it important that the alcohol…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Why is it important that the alcohol used in the DNA extraction is kept cold?

1.Helps to precipitate the DNA in the alcohol layer, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield.

2.Helps to precipitate the DNA in the aqueous layer, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield.

3.Helps to precipitate the proteins at the interface between the aqueous and alcohol layers, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield.

4.Helps to precipitate the DNA at the interface between the aqueous and alcohol layers, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield.

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