3. You isolate DNA from 1 ml of a suspension of E. coli cells using the procedure outlined in your lab handout. After completing the DNA isolation procedure, the total volume of the DNA solution is 600 ul. You transfer 10 µl of this DNA solution into 490 µl of TE buffer to create a diluted DNA solution. Using a spectrophotometer, you determine that the concentration of the diluted DNA solution is 245 µg/ml. a. How much did you dilute the original DNA solution? Express the dilution as a ratio. b. What is the concentration of the original DNA solution? C. How much total DNA did vou isolate from the suspension of F. coli cells?
3. You isolate DNA from 1 ml of a suspension of E. coli cells using the procedure outlined in your lab handout. After completing the DNA isolation procedure, the total volume of the DNA solution is 600 ul. You transfer 10 µl of this DNA solution into 490 µl of TE buffer to create a diluted DNA solution. Using a spectrophotometer, you determine that the concentration of the diluted DNA solution is 245 µg/ml. a. How much did you dilute the original DNA solution? Express the dilution as a ratio. b. What is the concentration of the original DNA solution? C. How much total DNA did vou isolate from the suspension of F. coli cells?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Transcribed Image Text:3. You isolate DNA from 1 ml of a suspension of E. coli cells using the procedure outlined in your
lab handout. After completing the DNA isolation procedure, the total volume of the DNA
solution is 600 ul. You transfer 10 µl of this DNA solution into 490 µl of TE buffer to create a
diluted DNA solution. Using a spectrophotometer, you determine that the concentration of the
diluted DNA solution is 245 ug/ml.
a. How much did you dilute the original DNA solution? Express the dilution as a ratio.
b. What is the concentration of the original DNA solution?
c. How much total DNA did you isolate from the suspension of E. coli cells?
Expert Solution
Step 1
a. 10 μl DNA solution was added to 490 μl buffer to make a total volume of 500 μl.
Therefore the dilution is 10:490 or 1:49 (v/v DNA:buffer).
b. 10 μl of DNA solution (total volume 500 μl) has a concentration of 245 μg/ml
Using, the formula, C1V1=C2V2 (C1= concentration of original DNA solution, V1=10 μl, C2= 245 μg/ml, V2=500 μl)
=> C1 x 10 μl= 245 μg/ml x 500 μl
=> C1 = (245 μg/ml x 500 μl)/ 10 μl
=> C1 = 12250 μg/ml or 12.25 mg/ml
Therefore, the concentration of original DNA solution was 12250 μg/ml or 12.25 mg/ml.
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