9. Estimate the sizes of the bands, in each of the lanes for the gel attached below. Then, for each lane, match the fragments created with the set of restriction enzymes used in the digestion of 640 bp DNA.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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9. Estimate the sizes of the bands, in each of the lanes for the gel attached below. Then, for each lane, match the fragments created with the set of restriction enzymes used in the digestion of 640 bp DNA.

Base Pairs
1,000
900 -
800 -
700 -
600 -
500 -
400 -
300 -
200 -
100 -
50 -
Enzyme sets:
• AB: Avrll & Bglll
E: ECORI & ECORV
F: Fatl
• H: Hindll & HpyCH4V
• KNP: Kpnl & Ncol & Pmll
• X: Xbal & Xhol
1 2
3
4
5 6
M Base Pairs
-1,517
-1,200
-1,000
006-
-800
- 700
-600
-500/517
-400
-300
-200
- 100
-50
Saved
Normal
BIIIU
fx I D
B ELUT 8 E lli
Lane 1
Lane 2
Lane 3
Lane 4
Lane 5
Lane 6
Band 1 size
Band 2 size
Band 3 size
Band 4 size
Band 5 size
Restriction enzymes used
DNA ladder
Paper DNA digest
Transcribed Image Text:Base Pairs 1,000 900 - 800 - 700 - 600 - 500 - 400 - 300 - 200 - 100 - 50 - Enzyme sets: • AB: Avrll & Bglll E: ECORI & ECORV F: Fatl • H: Hindll & HpyCH4V • KNP: Kpnl & Ncol & Pmll • X: Xbal & Xhol 1 2 3 4 5 6 M Base Pairs -1,517 -1,200 -1,000 006- -800 - 700 -600 -500/517 -400 -300 -200 - 100 -50 Saved Normal BIIIU fx I D B ELUT 8 E lli Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Band 1 size Band 2 size Band 3 size Band 4 size Band 5 size Restriction enzymes used DNA ladder Paper DNA digest
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