36.  When choosing restriction enzymes for cloning a gene of interest into a plasmid, what is an important requirement? Group of answer choices The gene of interest must code for the restriction enzymes which will cleave the sequence The restriction enzyme cleavage site must overlap with a region in the gene of interest The length of the restriction enzyme cleavage site must be 6 nucleotides The restriction enzyme cleavage site must not be present in the gene of interest or antibiotic resistance marker

Q: How is bacterial DNA safe from digestion by Restriction Endonucleases?     The plasmid of the…

A: Question-  How is bacterial DNA safe from digestion by Restriction Endonucleases?   A) The…

Q: ased on the knowledge you gained from the cloning module, which of the lanes in the urs is…

A: Agarose gel electrophoresis is used to separate DNA molecules based on their size and analyze DNA…

Q: Explain how the SNP changes the recognition sequence for the restriction enzyme and affects its…

A: *Single nucleotide polymorphisms (SNPs) involve variation of a single base pair and or SNPsbin the…

Q: 83. A plasmid is cut with the restriction enzyme BamH1 giving fragments of 3000 and 1000 bp as…

A: In this plasmid, we got 2 fragments of length 1000 and 3000 bp after digestion with BamH1 that…

Q: 5. You wish to map restriction sites in an unknown plasmid that has not been sequenced. You digest…

A: The process of fragmenting deoxyribonucleic acid strands with specific enzymes is restriction…

Q: Probe Loss of restriction site Insertion 4 Deletion> The smallest RFLP generated would be associated…

A: Introduction :  RFLP ( restriction fragment length polymorphism ) is an first generation molecular…

Q: Site-directed mutagenesis involves: (MARK ALL THAT APPLY) Group of answer choices Joining two DNA…

A:

Q: This plasmid is digested with the enzymes listed below. Indicate how many fragments will be…

A:

Q: An RFLP can be created by any mutational event that cretes or deletes a restriction enzyme…

A: Introduction: In the field of molecular biology, restriction fragment length polymorphism (RFLP) is…

Q: 24. The diagram below shows the map for EcoRI restriction sites around the BRCA1 gene for two…

A: Answer well explained above.

Q: Write the 3' to 5' DNA bottom strand • Write the mRNA sequence of top strand Write the mRNA sequence…

A: In DNA   there are four nitrogen as base that is adenine, Thymine, guanine and cytosine is present.…

Q: Using the chart posted to the left of the microwave in lab, what is the 3. restriction recognition…

A: Step 1 In 1963, Arber discovered that Escherichia coli is able to protect itself from the attack of…

Q: 4. Animation 12.4: This table shows results from a genetic test conducted on DNA samples from two…

A: Restriction enzymes are the enzymes capable of cutting double-helical segments that contain a…

Q: When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with…

A: A plasmid is a double-stranded DNA molecule that is small and circular in shape.  Bactria and yeast…

Q: 25. The restriction enzymes Kpnl and Acc65l recognize and cleave the same 6-bp sequence. You have a…

A: Restriction enzymes are the DNA digesting enzymes.

Q: please answer these questions for this image What is the source of the DNA? What is the target?…

A: The objective of the question is to understand the process of genetic engineering, specifically the…

Q: Dr. Doom has a DNA sequence of 2000 bp. Enzyme X and Enzyme Y restriction sites and their positions…

A: Restriction enzymes are the enzymes which cut DNA at particular site.

Q: What is the most accurate way to introduce mutations to particular regions in the genome? Using…

A: An modification to the genome's nucleotide sequence of an organism, virus, extrachromosomal DNA, or…

Q: Restriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the…

A: Restriction mapping is a technique to map unknown segment of DNA by breakung it into small…

Q: Important elements of a directed evolution experiment (“evolution in a test tube”) for an…

A: The goal of sciences is to create control over molecular structures and their reactivity. The…

Q: You are considering using restriction enzymes that recognizes 4-bp and 6-bp nucleotide sequences,…

A: From theory we know, the longer the restriction site recognized by a particular restriction…

Q: In an restriction enzyme experiment where Eco RI and  Hind III are used . What would happen if a…

A: Introduction  An isolated bacterial protein known as a restriction enzyme cleaves DNA at…

Q: b. After molecular cloning and confirming you properly cloned stripes into the vector shown, you…

A: The goal of this study is to investigate the structure of the protein product STRIPES, which…