5. You wish to map restriction sites in an unknown plasmid that has not been sequenced. You digest the plasmid with combinations of EcoRI, SalI, and BamHI, producing fragment sizes as indicated in the table below. Draw a restriction map of the plasmid, indicating relative positions and distances between restriction sites. Restriction Enzyme(s) used | Resulting Fragment Sizes EcoRI 5386 Sall 5386 ВатHI 1079, 4307 3078, 2308 331, 1079, 3976 898, 1079, 3409 EcoRI + SalI ЕcoRI + BamНІ SalI + BamHI
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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