The restricition EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3' the restriction enzyme Hindll cleaves at the sequence 5'-AAGCTT-3'. A 20 kb circular plasmid containing OFP gene is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined using electrophoresis Restriction Enzyme(s) ECORI alone Fragment length 6 kb ,14 kb 7 kb , 13 kb 2kb, 4kb, 5 kb, 9kb Hindlll alone EcoRI and Hindll Construct the circular molecule and indicate the relative positions of the EcoRI and Hindlll restriction sites

Q: Restriction endonuclease digestion of a DNA sequence yielded fragments of the following sizes: 1.…

A: The objective of the question is to determine the order of DNA fragments after gel electrophoresis…

Q: 36.  When choosing restriction enzymes for cloning a gene of interest into a plasmid, what is an…

A: Restriction enzyme:           An enzyme that cuts dsDNA into fragments after recognising specific…

Q: you carry out DNA editing using CRISPR whter the editing template DNA has strands labeled with heavy…

A: DNA (Deoxyribonucleic acid) is edited with the help of several molecular tools. CRISPR (Clustered…

Q: PCR primers Below is a 300 base pair fragment of DNA. The top strand is written in the 5' to 3'…

A: The PCR is known as a polymerase chain reaction in which the DNA template is amplified with the help…

Q: etion enzyme Sall cuts the sequence GTCGAC to leave a five base, 5' overhang. The restriction enzyme…

A: A restriction enzyme is classified under the category of a protein. These are isolated from bacteria…

Q: Do virtual restriction digests of 300ng pGLO plasmid using HinDIII, EcoRI, and XhoI separately, and…

A: DNA restriction enzymes cleaves the DNA at specific sites. These recognition sites have DNA…

Q: A virus with a circular double-stranded DNA chromosome contains approximately 10,000 bp. You want to…

A: To construct a map of the viral chromosome based on the cleavage sites of the restriction…

Q: This is a restriction map for the 250 base pair plasmid pSage. Restriction sites for the restriction…

A: Restrictions endonucleases are enzymes that cut down the DNA into fragments near or at the site of…

Q: Fill in the blank spaces by selecting the correct enzyme for each of the restriction enzyme…

A: DNA  is cleaved to form DNA fragments with the help of an enzyme known as the restriction enzyme.…

Q: A student wanted to load .75 ug dna on agarose gel and has 4x loading buffer for sample preparation.…

A: Agarose gel electrophoresis is a method used for the separation of DNA fragments which is stained by…

Q: When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with…

A: A plasmid is a double-stranded DNA molecule that is small and circular in shape.  Bactria and yeast…

Q: Which of the following would allow for control of a gene's orientation when inserted into a plasmid…

A: The technique of molecular biology used to generate similar copies of a particular segment (gene) of…

Q: dont provide handwriting solution ...

A: When dealing with circular DNA containing multiple EcoRI recognition sites, the digestion pattern…

Q: These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and…

A: These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and…

Q: How many DNA fragments will result when the double-stranded DNA molecule below is digested with…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: 25. The restriction enzymes Kpnl and Acc65l recognize and cleave the same 6-bp sequence. You have a…

A: Restriction enzymes are the DNA digesting enzymes.

Q: pls help very urgent, no explaantion required, pls help asap

A: In molecular biology, a restriction digest is a technique for preparing DNA for analysis or further…

Q: The recognition sequence for the restriction enzyme Sau3AIis GATC; in the recognition sequence…

A: Given; The recognition sequence for the restriction enzyme Sau3AI is GATC. The recognition sequence…

Q: Primers designing for epitope tagging: Design forward and reverse primers to amplify the following…

A: Primers should generally have the following properties: 1. Length of 18-24 bases. 2. 40-60% G/C…

Q: Above are the results of gel electrophoresis following digestion with restriction enzymes. What is…

A: Using specialized enzymes known as restriction endonucleases, restriction digestion is a laboratory…

Q: Which of the following scenarios would ONLY occur if your skipped the digest purification step?…

A: Restriction enzymes are enzymes that cleave DNA at specific sites, and are commonly used in…

Q: How many fragments and how big each fragment should be after digesting the plasmid with BamHI? How…

A: Digestion of the pUC19 plasmid using the restriction enzymes BamHI and PvuI.1.BamHI Digestion:BamHI…

Q: is shown below. Draw a restriction map of a plasmid with the appropriate restriction sites based on…

A: Restriction digestion is the fragmentation(cutting) of DNA on very specific sites (restriction…

Q: Which of the following statement is true? * Restriction enzyme binds to non-cognate DNA better than…

A: Restriction enzymes also known as restriction endonucleases are enzymes that recognize and cleave…

Q: You utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using…

A: Bacteria produce the restriction enzyme, a protein that cleaves DNA at certain locations.  The…

Q: Assume complete digestion of a single linear piece of DNA digested (cut) with EcoRI and Hindill.…

A: A plasmid is a single-stranded, circular DNA molecule that is different from the chromosomal DNA of…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.