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2. Your Genetics professor gives you and your classmate's information on a restriction digest. The restriction enzymes and their respective cuts have been provided to you, as well as a cryptic restriction map. It is your job to determine what cuts (A, B, C) came from which restriction enzyme and what fragment sizes (a, b, c, d) belong in what order. Answer the following questions below using the information provided. Note: the restriction enzyme cuts are not to scale. Restriction Enzyme 1: 165, 413, 710 Restriction Enzyme 2: 511, 777 Double Digest (1 + 2): 165, 199, 413, 511 a) Indicate the restriction enzyme that made the cut at each location (A, B, and C). Note: A restriction enzyme may have cut the fragment at more than one location. b) Indicate the DNA fragment size from the double digest that belongs to each position (a, b, c, d) on the restriction map. As you will note, the size of each fragment does not identify the size of the fragment (the map is not to scale).

2. Your Genetics professor gives you and your classmate's information on a restriction digest. The restriction enzymes and their respective cuts have been provided to you, as well as a cryptic restriction map. It is your job to determine what cuts (A, B, C) came from which restriction enzyme and what fragment sizes (a, b, c, d) belong in what order. Answer the following questions below using the information provided. Note: the restriction enzyme cuts are not to scale. Restriction Enzyme 1: 165, 413, 710 Restriction Enzyme 2: 511, 777 Double Digest (1 + 2): 165, 199, 413, 511 a) Indicate the restriction enzyme that made the cut at each location (A, B, and C). Note: A restriction enzyme may have cut the fragment at more than one location. b) Indicate the DNA fragment size from the double digest that belongs to each position (a, b, c, d) on the restriction map. As you will note, the size of each fragment does not identify the size of the fragment (the map is not to scale).

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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2. Your Genetics professor gives you and your classmate's information on a restriction digest. The restriction enzymes and their respective cuts have been provided to you, as well as a cryptic restriction map. It is your job to determine what cuts (A, B, C) came from which restriction enzyme and what fragment sizes (a, b, c, d) belong in what order. Answer the following questions below using the information provided. Note: the restriction enzyme cuts are not to scale. Restriction Enzyme 1: 165, 413, 710 Restriction Enzyme 2: 511, 777 Double Digest (1 + 2): 165, 199, 413, 511 a) Indicate the restriction enzyme that made the cut at each location (A, B, and C). Note: A restriction enzyme may have cut the fragment at more than one location. b) Indicate the DNA fragment size from the double digest that belongs to each position (a, b, c, d) on the restriction map. As you will note, the size of each fragment does not identify the size of the fragment (the map is not to scale). A B d + a
Transcribed Image Text:2. Your Genetics professor gives you and your classmate's information on a restriction digest. The restriction enzymes and their respective cuts have been provided to you, as well as a cryptic restriction map. It is your job to determine what cuts (A, B, C) came from which restriction enzyme and what fragment sizes (a, b, c, d) belong in what order. Answer the following questions below using the information provided. Note: the restriction enzyme cuts are not to scale. Restriction Enzyme 1: 165, 413, 710 Restriction Enzyme 2: 511, 777 Double Digest (1 + 2): 165, 199, 413, 511 a) Indicate the restriction enzyme that made the cut at each location (A, B, and C). Note: A restriction enzyme may have cut the fragment at more than one location. b) Indicate the DNA fragment size from the double digest that belongs to each position (a, b, c, d) on the restriction map. As you will note, the size of each fragment does not identify the size of the fragment (the map is not to scale). A B d + a
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