How many restriction fragments did the digest produce in Lane 3? What does that mean about the number of restriction sites that species had in its DNA? b. Provide your estimates for the lengths of the restriction bands in Lane 3.

Q: a. What are the purposes of sequence alignment?

A: Sequence alignment is a method by which one can arrange sequences of DNA, RNA and protein so as to…

Q: What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of…

A: Restriction fragment length polymorphism (RFLP) : it is a method used in genome mapping and…

Q: Plasmid is  3.6kb large. It is digested with an enzyme that has restriction sites at  1,152 and…

A: Plasmids are circular DNA found in bacteria. They are extra chromosomal materials and are highly…

Q: Explain how the SNP changes the recognition sequence for the restriction enzyme and affects its…

A: *Single nucleotide polymorphisms (SNPs) involve variation of a single base pair and or SNPsbin the…

Q: Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the…

A: Vast uses of endonuclease activity of restriction enzyme found in the biotechnology for gene…

Q: Match the term with its description.

A: There are various enzymes and gene components that are studied in techniques of genetic engineering.…

Q: How would the results of this activity have been different if the DNA sequence you digested were…

A: Gel electrophoresis is a process in which DNA fragments are separated on the basis of their size.…

Q: The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme…

A: In molecular biology procedures, restriction enzymes are especially important for DNA cloning. They…

Q: BamHI Haelll Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 How long is the original DNA molecule?

A: If it is a closed circular DNA, BamH1  cut it into three fragments of length 10kb 5kb 2kb

Q: Why does Northern blotting not require the use of restriction enzymes?

A: Restriction enzyme naturally cleaves DNA because it's have double stranded sequence of neucleiotides…

Q: QUESTION 24 Answer Assume that a given plasmid vector to be used in a cloning experiment contains…

A: Plasmid is a circular dna molecule used in cloning experiments. This is a small double stranded DNA…

Q: Draw a diagram showing what pGEM will look like after it has been digested with BamHI.

A: BamHI: BamHI is a restriction enzyme obtained from Bacillus amyloliquefaciens that specifically cuts…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Restriction enzymes These are proteins that are isolated from the bacteria that cleave the sequences…

Q: Compatible ends generated by restriction enzyme can be connected by ligase. Determine which of the…

A: Restriction enzymes are proteins that cut DNA at specific sequences. These sequences are usually…

Q: Which of the following would allow for control of a gene's orientation when inserted into a plasmid…

A: The technique of molecular biology used to generate similar copies of a particular segment (gene) of…

Q: dont provide handwriting solution ...

A: When dealing with circular DNA containing multiple EcoRI recognition sites, the digestion pattern…

Q: Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is…

A: Plasmid:Circular mostlySize of plasmid is between <5kb - >200kbMay or may not be present in…

Q: a. Why doesn't Dpnl digest the mutagenized DNA?  b.What is the approximate size, in base pairs, of…

A: a. Why doesn't Dpnl digest the mutagenized DNA?  b.What is the approximate size, in base pairs, of…

Q: Calculate the average sizes and numbers of DNAfragments produced by digesting human genomic DNAwith…

A: DNA is the genetic material of many eukaryotic and prokaryotic organisms. It is a double-stranded…

Q: State the characteristics that would be required for using a restriction endonuclease with human…

A: Restriction enzymes or restriction endonuclease are the enzyme naturally present in the bacteria…

Q: After selection on ampicillin media, other diagnostic tools would be used to validate which of the…

A:

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.