How many restriction fragments did the digest produce in Lane 3? What does that mean about the number of restriction sites that species had in its DNA? b. Provide your estimates for the lengths of the restriction bands in Lane 3.

Q: 1.  (a) Restriction sites are usually ______.     Recombinant DNA Technology     Restriction…

A: Given, 1.  (a) Restriction sites are usually ______.     Recombinant DNA Technology…

Q: Some restriction endonucleases produce blunt-ended pieces of DNA, while other produce DNA fragments…

A: DNA (deoxyribonucleic acid) is the unit of an organism. The DNA is inherited from the parent…

Q: 5.Statement 1: Restriction enzymes are sequence-specific, which means that they only recognize a…

A: Restriction enzymes also known as restriction endonucleases are the DNA cutting enzymes that are…

Q: Describe What are the sticky ends of the restriction fragments? Select one: a. The surfaces of…

A: Restriction endonuclease are enzymes that help divide DNA and generate small pieces in molecular…

Q: A linear piece of DNA that is 14 kb long is cut first by EcoRI alone, then by SmaI alone, and…

A: Restriction enzymes are the endonucleases that digest the DNA at specific sites called restriction…

Q: 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would…

A: As per our guidelines, we are supposed to answer only one question. Kindly repost the other question…

Q: Specify the method for Purification of DNA fragment after digestion.

A: Dna is the polymer formed by monomer units called nucleotides . The dna molecule when degraded…

Q: Draw a diagram showing what pGEM will look like after it has been digested with BamHI.

A: BamHI: BamHI is a restriction enzyme obtained from Bacillus amyloliquefaciens that specifically cuts…

Q: Assuming a circular piece of DNA (plasmid) was used as starting material, how many restriction sites…

A: Restriction enzymes are specific endonuclease that can cut a DNA at a particular sequence.…

Q: Mention two classes of restriction enzymes. Suggest their respective roles.

A: The enzyme mainly involved in recombinant DNA technology are restriction enzymes, ligases, lyases,…

Q: Construct a detail linear restriction map according to the given information in Table 1 below. Table…

A: Total size of DNA= 11 kb EcoRI has one restriction site cutting it into 2 fragments of 2kb and 9kb…

Q: Which two restriction enzymes where used to completely digest the DNA fragment above based on the…

A: Restriction endonucleases are the enzymes which cleave the DNA at specific sites from within and…

Q: Assume that a circular plasmid is 3200 base pairs in length and has restriction sites for HindIII…

A: HindIII is a Haemophilus influenzae type II site-specific deoxyribonuclease restriction enzyme that…

Q: You have a DNA sequence of 1400 bp. To characterize it, you decide to make a restriction map.…

A: DNA is a nucleotide polymer present in living beings.

Q: The same restriction endonuclease must be used to excise the foreign DNA and bacterial DNA. Select…

A: DNA or deoxyribonucleic acid is a double-stranded molecule, strands of which are coiled around one…

Q: What normal role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from…

A: A restriction enzyme is a protein that recognizes a particular DNA sequence and cuts the DNA at a…

Q: Consider a partial restriction digestion, in which genomic DNA is exposed to a small, limiting…

A: The process of cutting or cleaving DNA by using restriction enzymes is called digestion or…

Q: Identify the basic steps (in right order) in AFLP marker analysis. Explain briefly how this marker…

A: Basic steps in AFLP marker analysis.

Q: A DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the…

A: DNA fingerprinting is commonly employed in investigations to connect evidence with suspects, but DNA…

Q: What are the sticky ends of the restriction fragments?   Select one: a. The surfaces of sticky ends…

A: In molecular biology , Restriction endonuclease are the enzymes that helps in splitting the DNA and…

Q: If a uidA amplicon generateed by PCR is 200bp and the DNA fragments resulting from the restriction…

A: INTRODUCTION Polymerase Chain reaction This is the method of amplification of of nucleic acids such…

Q: Consider a partial restriction digestion, in which genomic DNA is exposed to a small, limiting…

A: The process of cutting or cleaving DNA by using restriction enzymes is called digestion or…

Q: Please asap Original DNA template: 3'-ACGGTCAATTTGCTG-5 a) Transcribe the sequence. b) Translate the…

A: Given: Original DNA template: 3' - ACGGTCAATTTGCTG - 5'

Q: Dr. Doom has a DNA sequence of 2000 bp. Enzyme X and Enzyme Y restriction sites and their positions…

A: Restriction enzymes are the enzymes which cut DNA at particular site.

Q: molecular scissors", justify that

A: Restriction enzymes are found in a wide variety of prokaryotes. Restriction enzymes received their…

Q: Compare and contrast the following terms: cDNA and gene Restriction fragment and gene DNA probe and…

A: Gene expression is a process in which the genetic instructions of genes are utilized to manage…

Q: Restriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the…

A: Restriction mapping is a technique to map unknown segment of DNA by breakung it into small…

Q: Explain the Restriction Enzymes ?

A: An enzyme derived from microbes that breaks DNA molecules at specified sequences is known as a…

Q: restriction enzyme

A:

Q: If pET32a(+) is digested simultaneously with BamHI and BclI, 1.How many fragments are created? 2.…

A: Cells contain nucleic acid which stores essential genetic information for the survival of the cell.…

Q: If the following is a restriction enzyme: Sma I, A. What is the first letter represent? B. What do…

A: Sma I is a restriction enzyme. It cuts the DNA. The recognition sequence it recognizes is CCCGGG.

Q: f restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up…

A: Nucleases are enzymes that degrade nucleic acids. They can be classified into RNase   : enzymes…

Q: The activity of restriction enzymes may produce fragments with sticky ends.  Sticky ends are   a) a…

A: Correct option is (c). Restriction enzyme, also called as restrictions endonucleases,  is a…

Q: If the Hinfl restriction enzyme recognizes G^ACTC sites, as seen in the image, belUW. GACTC How many…

A: The cloning of DNA is the mechanism by which a certain fragment of DNA is multiplied. The selected…

Q: Describe the process for shotgun sequencing of a genome. Practice aligning the two sets of sequenced…

A: Since you have posted multiple questions, we solve the first question for you. To get the remaining…

Q: For the given restriction enzyme: BslI (CCNNNNN^NNGG)  i) Approximately how many fragments would…

A: Restriction endonucleases are enzymes that does cleavage of DNA at specific sites. These specific…

Q: A 10 kb DNA fragment digested with the restriction endonuclease EcoRI yields fragments of 4 kb and 6…

A: Restriction enzymes are also known as restriction endonucleases. These are the enzymes produced by…

Q: 1. A linear DNA molecule is subjected to complete restriction digestion by (1) EcoR1 alone, (2)…

A: Restriction enzyme or restriction endonuclease are bacterial protein that cleaves DNA at specific…

Q: Assume that a circular plasmid is 3200 base pairs in length and has restriction sites for HindIII…

A: Restriction endonucleases are the enzymes which recognize and cleaves DNA to make smaller fragments.…

Q: Please answer both parts, a. The enzyme that catalyzes the joining of fragments to form recombinant…

A: The recombinant DNA technology is a  branch of biology that deals with different techniques that…

Q: Restriction enzymes cut double-stranded DNA _____.   a. at noncoding areas between genes   b.…

A: Restriction enzymes cut double-stranded only at specific sequence of the dna, producing either a…

Q: The total size of the plasmid is 2686 bp. There is a PstI recognition site at position 439, HindIII…

A: Answer - d. 3 fragments: 447bp, 507bp and 1732bp

Q: What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger…

A: Since you have posted a question with multi- sub parts , we will solve first three sub-parts for…

Q: How does that length compare to that of restriction enzymes? Implications?

A: Answer- Restriction enzyme is also called as molecular sisscors because it is used to cut the DNA…

Q: b) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis…

A: DNA digestion is the process in molecular biology which is done by restriction enzymes for the…

Q: a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short…

A: Note: Solving answer only for part A as per the guidelines, repost remaining part, please. The…

Q: Based on the banding pattern and constructed map which of the following statements is TRUE? O A. The…

A: Restriction mapping is a way of locating and identifying the unknown segments of  DNA by digesting…

Q: If insert DNA was obtained by PCR using Taq DNA polymerase, what shape does the end of this DNA…

A: Taq polymerase is basically a thermostable polymerase that is obtained from the bacteria called as…

Q: 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would…

A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…

Q: A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel…

A: The breaking apart or fragmentation of DNA strands is known as DNA fragmentation. It might be done…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.