How many restriction fragments did the digest produce in Lane 3? What does that mean about the number of restriction sites that species had in its DNA? b. Provide your estimates for the lengths of the restriction bands in Lane 3.

Q: linkage disequilibrium

A: a) Linkage:  Linkage is the phenomenon of certain genes staying together during inheritance…

Q: a. What are the purposes of sequence alignment?

A: Sequence alignment is a method by which one can arrange sequences of DNA, RNA and protein so as to…

Q: What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of…

A: Restriction fragment length polymorphism (RFLP) : it is a method used in genome mapping and…

Q: 2- Supposed that you have a DNA sequence sample with 10,000 bp. By using 2 different restriction…

A: The answer is 3.

Q: Design PCR primers for the DNA sequence given below and explain your choice. As part of your answer,…

A: It is a Polymerase Chain Reaction, which is a mechanism for multiplying a specific piece of DNA…

Q: Explain how the SNP changes the recognition sequence for the restriction enzyme and affects its…

A: *Single nucleotide polymorphisms (SNPs) involve variation of a single base pair and or SNPsbin the…

Q: a. What sequence information about a gene is lackingin a cDNA library?b. Can clones in a cDNA…

A: The cDNA or complementary DNA is derived from mRNA which is transcribed from genomic DNA (gDNA).  It…

Q: Match the term with its description.

A: There are various enzymes and gene components that are studied in techniques of genetic engineering.…

Q: (RE) recognition sites marked with arrows. A spontaneous mutation (insertion) occurred in DNA sample…

A: Answer ::

Q: 10. Based on the restriction map of the above plasmid, determine the number of DNA fragment(s) and…

A: Restriction enzyme digestion is a crucial process in molecular biology that enables the cleavage of…

Q: How would the results of this activity have been different if the DNA sequence you digested were…

A: Gel electrophoresis is a process in which DNA fragments are separated on the basis of their size.…

Q: The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme…

A: In molecular biology procedures, restriction enzymes are especially important for DNA cloning. They…

Q: BamHI Haelll Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 How long is the original DNA molecule?

A: If it is a closed circular DNA, BamH1  cut it into three fragments of length 10kb 5kb 2kb

Q: B) Suppose, you had a linear DNA instead of circular DNA. The double stranded sequence of a…

A: Introduction :- Restriction enzymes, also known as restriction endonucleases, are enzymes that can…

Q: b. The diagram below is of a short stretch of prokaryotic chromosomal DNA in the process of…

A: Replication It is defined as the process in which the DNA duplicates into another copy which is…

Q: Why does Northern blotting not require the use of restriction enzymes?

A: Restriction enzyme naturally cleaves DNA because it's have double stranded sequence of neucleiotides…

Q: You wish to perform an electrophoretic resolution of your restriction enzyme–digested DNA. The sizes…

A: Note: As per the guidelines we are answering the first question here. Please repost the other…

Q: Examine the sequence for the DNA fragment below. Your job is to design primers for PCR that would be…

A: A primer may be a brief single-stranded nucleic acid utilized by all living living beings within the…

Q: Compatible ends generated by restriction enzyme can be connected by ligase. Determine which of the…

A: Restriction enzymes are proteins that cut DNA at specific sequences. These sequences are usually…

Q: Assume that a circular plasmid is 3200 base pairs in length and has restriction sites for HindIII…

A: HindIII is a Haemophilus influenzae type II site-specific deoxyribonuclease restriction enzyme that…

Q: 1) The DNA fragment shown in Figure 1 is cleaved by the restriction enzyme EcoRI as indicated. The…

A: Q 1.) :- Answer.Based on the given information, the DNA fragment shown in Figure 1 is cleaved by the…

Q: Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is…

A: Plasmid:Circular mostlySize of plasmid is between <5kb - >200kbMay or may not be present in…

Q: 500 9. The restriction site sequence for the restriction enzyme Sau3AI and BamHI include four…

A: Introduction:- Restriction endonucleases are the bacterial enzymes that cuts double stranded DNA…

Q: a. The reading frame DNA sequence is  b. The mRNA sequence is  c. The polypeptide sequence is…

A: A "nucleic acid" is a linear polymer of nucleotides that is a component of the cell's information…

Q: Calculate the average sizes and numbers of DNAfragments produced by digesting human genomic DNAwith…

A: DNA is the genetic material of many eukaryotic and prokaryotic organisms. It is a double-stranded…

Q: restriction enzyme

A:

Q: 1. A DNA sequence 10 kb long contains one site at which it may be cut by the restriction enzyme…

A: Restriction enzymeIt refers to the enzyme that recognizes specific DNA sequences and cleaves the DNA…

Q: After selection on ampicillin media, other diagnostic tools would be used to validate which of the…

A:

Q: Which of the following statement is true? * Restriction enzyme binds to non-cognate DNA better than…

A: Restriction enzymes also known as restriction endonucleases are enzymes that recognize and cleave…

Q: You have another circular plasmid. Complete and effective digestion of this plasmid with a…

A: The restriction enzymes are endonuclease that means they can cut the DNA from the middle. They are…

Q: a. what is the nucleotide sequence of the DNA chain synthesized from the primer. label the 5' to 3'…

A:

Q: A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid…

A: Plasmids are small, circular, double stranded, and extrachromosomal bacterial DNA. i.e. it is…

Q: What is the final volume of the individual PCR reactions we are making?

A: Polymerase chain reaction (PCR) is an in vitro method of making large number of copies of desired…

Q: DNA ladder Sample digested with EcoRI negative electrode 23- This is the result of gel…

A: Introduction Gel electrophoresis is a technique that uses size and charge to separate and analyse…

Q: A circular DNA molecule is subjected to complete restriction digestion by (1) BamHI alone, (2)…

A: Restriction enzymes cut DNA at a specific location called as restriction site.

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.