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need to understand how to know which is 5' overhand and which is 3' overhang

need to understand how to know which is 5' overhand and which is 3' overhang

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Can you please answer this questions with details, I need to understand how to know which is 5' overhand and which is 3' overhang, thank you. ( Question attached)

We use one restriction endonuclease (RE), which cuts after G (as shown by red arrow) 5'GAATTC3' 3'CTTAAGS' 1. After cut, how do the ds DNA strands look like? 2. What are these ends called? 3. Is it a 5' or 3' overhang?
Transcribed Image Text:We use one restriction endonuclease (RE), which cuts after G (as shown by red arrow) 5'GAATTC3' 3'CTTAAGS' 1. After cut, how do the ds DNA strands look like? 2. What are these ends called? 3. Is it a 5' or 3' overhang?
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