24. The diagram below shows the map for EcoRI restriction sites around the BRCA1 gene for two alleles of the gene, andthese alleles. The DNA was digested with the restriction enzyme EcoRI and probed with a radioactively labeled DNA froma cloned copy of the BRCA1 gene. Which individual or individuals are homozygous for allele 1?a southern blot of DNA for four people that are either homozygous for one of the two alleles or are heterozygous foSouthern Blot12 34Allele 1Allele 2BRCA1EcoRIEcoRIEcoRI10 kbLA) 1B) 2 and 3C) 412 kbD) 1 and 4E) You cannot identify homozygous individuals without data for the offspring of these individuals.==

Answered: 24. The diagram below shows the map for…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

Bacterial systems serve as an excellent model to express proteins but has a disadvantage – what is that disadvantage? List the points to differentiate three classes of restriction enzymes? Give an example of restriction enzyme that has ability to generate blunt and cohesive ends after digestion of DNA?
The human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted with hemophilia. The man with blackened symbol (III-1) has hemophilia. To help with genetic diagnosis, a probe that detects an RFLP (restriction fragment length polymorphism) on the X chromosome is used. This probe detects either a 7 kb restriction enzyme fragment or 3 kb and 4 kb restriction enzyme fragments. The RFLP pattern for all the members of the pedigree is shown. The recombination distance between the RFLP and the hemophilia locus is 40%. What is the chance that their unborn child (III-7) is affected? 4 kb 3 kb Select one: a. 10% b. 20% C. 40% d. 60% e. 80% f. 90% g. None of these above II II
PCHEM4321. An agarose gel electrophoresis pattern of the plasmid PSPM4321 digestion (restriction) is shown below. Draw a restriction map of a plasmid with the appropriate restriction sites based on the data given below. Hindlll Hindll BamHI +BamHI Figure 1: 1% agarose gel electrophoresis of pCHEM4321 40 24 16 12 12 8 4 4 + |
19. You run a PCR of the above HBB sequence using the primers you designed. You receive a vector from a company that you want to clone your HBB PCR product into. Below is the vector: BamHI 0.5 kb EcoRI 1.0 kb 0.3 kb -Bglll plasmid A 0.2 kb BamHI 0.4 kb HindIII 1.0 kb EcoRI You want to cut this vector with BamHI and EcoRI and insert your PCR product into this vector. After cutting the vector with both enzymes simultaneously you run a gel. i) On the gel below draw the bands you expect to see after you cut this vector with these enzymes at the same time (only use one gel lane - the other one is going to be empty). (6 points) Ladder 5kb 3kb 2.5kb 2kb 1.5kb 1kb 0.5kb 0.3kb 0.2kb ii) Circle the band that represents the backbone that you will insert the PCR product into. (2 points) c) Once you have inserted the PCR product into the plasmid backbone from part C, how many bp will the new vector be (the total of the backbone and the PCR product together). Answer should be 2 significant figures.…
A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic regions. The insect’s genomic DNA has been treated with the restriction enzyme EcoRI. One of the fragments (with a length of 4 kb) produced in this manner is isolated and ligated into a pUC19 plasmid vector that has also been treated with EcoRI. This recombinant construct is called plasmid A. Samples of plasmid A, pUC 19, and the total genomic DNA from the insect are digested completely with the restriction enzyme EcoRI. The three digests are loaded into separate wells: plasmid A (lane 1), pUC19 (lane2) and total insect genomic DNA (lane 3) of an agarose gel and subjected to electrophoresis. 2b. Draw what will be seen in each lane if the gel is transferred to a membrane and subjected to Southern blotting and the 4000 base pair genomic DNA fragment (labeled with a fluorescent tag) from the insect is used to probe the blot.
Please determine the relative location of the three restriction enzymes. Enzyme Number of fragments Size (kb) Xbal 2 17, 21 Xhol 2. 5, 33 Xbal/Xhol 5, 12, 21 Kpnl 6, 7, 58 Xbal/Kpnl 6, 7, 8, 17
Give typing answer with explanation and conclusion
b. After molecular cloning and confirming you properly cloned stripes into the vector shown, you transform bacteria with the plasmid and select out bacteria that contain the plasmid. Now, you want to these transformed bacteria to express the STRIPES. Under what conditions would we need to grow bacteria transformed with this plasmid so that they express STRIPES? Explain your answer, and be sure to describe the important regulatory regions on the plasmid above and what interacts with these regulatory regions in the conditions you have indicated.
A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA? a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb b. 2.0kb c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bp
Compared to the normal A allele, the disease-causing allele in sickle cell anemia (S allele) is missing an MstII restriction site. On a Southern blot of genomic DNA cut with MstII and hybridized with the probe shown on the diagram below, a person with sickle anemia, carrying two S alleles, will show Choose an answer below: a single band at 1.1 kb. a single band at 1.3 kb. a single band at 0.2 kb. one band at 0.2 and one at 1.3 kb. one band at 1.1 and one at 1.3 kb.
1) Using the diagram below, sketch in the pattern of bands you would expect to see after digesting the DNA of the TT, Tt and tt genotypes of the TAS2R38 gene. Use the Base Pair Standards on the left of the diagram as a reference in drawing the positions of the bands. Base Pair TASTER GENO TYPE Standards (Base Pairs) TT Tt tt 500 400 300 200 100 50
Which pair of restriction enzymes will you use to insert EGFP gene from Addgene Vector 174028 in Addgene Vector 81061 between EF1-alpha-intron A and PGK promoter? BsrGl and BamHI EcoRI and BsrGl EcoRI and Kpnl Kpnl and BstBl

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