3). You mutagenize bacteria, plate them to the permissive temperature, then replica plate to the restrictive temperature. After lots of replica plating and mapping, you find mutants in dnaB. a). What is the phenotype of these mutants at permissive and non-permissive temperatures? Focus on the macroscopic phenotype (what happens at the level of cells) and not the molecular phenotype (which step in replication is compromised). b). Can you make constitutive mutants (the mutation is always active) in dnaB? Why or why not?
3). You mutagenize bacteria, plate them to the permissive temperature, then replica plate to the restrictive temperature. After lots of replica plating and mapping, you find mutants in dnaB. a). What is the phenotype of these mutants at permissive and non-permissive temperatures? Focus on the macroscopic phenotype (what happens at the level of cells) and not the molecular phenotype (which step in replication is compromised). b). Can you make constitutive mutants (the mutation is always active) in dnaB? Why or why not?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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3. Please help answer this question and show all work, thank you so much.
Transcribed Image Text:3). You mutagenize bacteria, plate them to the permissive temperature, then replica plate to the
restrictive temperature. After lots of replica plating and mapping, you find mutants in dnaB.
a). What is the phenotype of these mutants at permissive and non-permissive
temperatures? Focus on the macroscopic phenotype (what happens at the level of cells)
and not the molecular phenotype (which step in replication is compromised).
b). Can you make constitutive mutants (the mutation is always active) in dnaB? Why or
why not?
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