Which pair of restriction enzymes will you use to insert EGFP gene from Addgene Vector 174028 in Addgene Vector 81061 between EF1-alpha-intron A and PGK promoter? BsrGl and BamHI EcoRI and BsrGl EcoRI and Kpnl Kpnl and BstBI

Q: Single stranded DNA fragment of M13 phage DNA shown was mixed with the following  primers P1: AGTAG…

A: Polymerase chain reaction also abbreviated as PCR.A specific DNA fragment is quickly multiplied…

Q: etion enzyme Sall cuts the sequence GTCGAC to leave a five base, 5' overhang. The restriction enzyme…

A: A restriction enzyme is classified under the category of a protein. These are isolated from bacteria…

Q: Comment on the failure of p1vir transduction experiments and give a reason why you think the…

A: transduction experiments is a technique commonly used in molecular biology to introduce foreign DNA…

Q: The following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a…

A: Given, conjugation occurs between an (a+d+g+) donor and an (a-d-g-) recipient.  Hence, parental…

Q: Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains…

A: Sickle cell anemia is a result of a mutation in the HBB gene. This gene contains the restriction…

Q: In this western blot, the levels of TBK remain constant with increasing amounts/expression of the…

A: TBK-1 gene encodes an enzyme known as TBK-1 (TANK binding kinase-1). This is a…

Q: It was suspected that the cause of the infertility seen in a patient was due to undiagnosed non-…

A: The question is asking us to determine the number of fragments that would be visible on an agarose…

Q: plate your cells on a master plate without any antibiotics.  After colonies appear, you pick and…

A: Recombinant DNA technology is helpful for different gene related experiments.

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: Introduction :- Recombinant DNA technology ( also known as genetic engineering) involves the use of…

Q: 18. You want to express hemoglobin beta (HBB) in a bacteria model using a vector. You would like to…

A: PCR (Polymerase Chain Reaction) is a laboratory technique used to amplify specific regions of DNA.…

Q: In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an…

A: Transduction is a process of gene transfer in which DNA (deoxyribonucleic acid) from a source is…

Q: 35) A researcher inserted DNA fragments from an organism into expression vector plasmids and…

A: A plasmid is a small, circular, extra double stranded molecule of DNA that is being different from…

Q: position of the Start and Stop. The GFP and RFP genes shown do not have start

A: The Cre-Lox system is a widely used tool in genetic engineering for site-specific modifications in…

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: A plasmid vector is a small, circular DNA molecule that is commonly used in molecular biology for…

Q: Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small…

A: As per the central dogma of biology, the flow of genetic information is from DNA -> RNA ->…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: You want to have E. coli take up your recombinant DNA vector you designed. What is the most likely…

A: A plasmid vector is a small, circular DNA molecule that is commonly used in molecular biology for…

Q: The genotypes of five merozygote bacteria are shown below. In each set, the enotype of the lac…

A: operon  Operon can be defined as a multiple gene that have only single promoter. All these genes are…

Q: When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with…

A: A plasmid is a double-stranded DNA molecule that is small and circular in shape.  Bactria and yeast…

Q: In organism that is mutant for components of the non-homologous end-joining NHEJ repair pathway…

A: CRISPR gene editing is a molecular biology genetic engineering tool for altering the genomes of…

Q: A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial…

A: The restriction endonuclease is the enzymes that help in the deduction of the DNA from a specific…

Q: Refer to the lac operon image above to see the available choices. Which lettered item represents the…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A:

Q: You are trying to clone you favorite piece of DNA into the PUC vector shown above. You've pulled an…

A: Joachim Messing and colleagues developed a variety of plasmid cloning vectors, including pUC19. The…

Q: The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence…

A: PCR is a polymerase chain reaction, this is a biotechnological process which is used to amplify the…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.