You want to have E. coli take up your recombinant DNA vector you designed. What is the mostlikely negative consequence of forgetting to add an antibiotic to the nutrient-containing plate thatyou plan to grow E. coli on?O Blunt ends, instead of sticky ends, will be formed in the vector and gene insert.O E. coli that did not take up your vector will grow on the plate.OThe restriction endonuclease will not be able to cut the vector or gene insert.No E. coli will grow on the plate.The gene of interest will not be properly ligated into the vector.

A plasmid vector is a small, circular DNA molecule that is commonly used in molecular biology for the cloning and manipulation of DNA sequences. Plasmids are able to replicate independently of the host cell's chromosomal DNA, and they can be easily introduced into bacterial or eukaryotic cells.Antibiotics are substances which either kill or inhibit the growth the bacterial cells. Antibiotics are used during molecular cloning experiments to select the clones in which the external DNA such as plasmid has been successfully inserted. The plasmid contains antibiotic resistance genes so the genes having the plasmid will be resistant to the antibiotic while cells which did not pick up the plasmid will be susceptible to antibiotic treatment.The most likely negative consequence of forgetting to add an antibiotic to nutrient containing plate that is to be used to grow E coli is that the E coli that did not take up the vector will grow on the plate.