You’re working in a research lab, and your current task is to clone the gene that codes for tyrosinase from potatoes. You grind up some potato, extracts the DNA from it and digests the DNA with two different restriction enzymes (separately, not together): EcoRI and BamHI. You then obtain the cloning vector, pUC19, and digest it with the same two enzymes. You then run a gel which is shown to the right.You mix the potato DNA (digested with the enzyme you specified in part B) with cloning vector DNA (digested with the same enzyme). You then add the mixture to E. coli cells and carry out a transformation procedure so that the cells can each update a plasmid. You then plate the cells on a plate containing antibiotic. What antibiotic would be in the plate? Why would there be antibiotic in the plate? Be specific!Unfortunately, you don’t get a single bacterial colony to grow on the plate. Not even one! You review your procedure and realize that when mixing the digested potato DNA and digested plasmid that you forget to add a critical enzyme. What enzyme needed to be added? Why? Be very specific in your explanation. OOO D O||IIII | | || 2500lacza396-454Polylinker500аmpPUC192686 bp2000ori10001500

Recombinant DNA technology is one of most significant techniques of genetic engineering. It enables…

Answered: You’re working in a research lab, and…

Medical Terminology for Health Professions, Spiral bound Version (MindTap Course List)

8th Edition

ISBN:9781305634350

Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder

Publisher:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder

Chapter5: The Cardiovascular Sytem

Section: Chapter Questions

Problem 93LE

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You’re working in a research lab, and your current task is to clone the gene that codes for tyrosinase from potatoes. You grind up some potato, extracts the DNA from it and digests the DNA with two different restriction enzymes (separately, not together): EcoRI and BamHI. You then obtain the cloning vector, pUC19, and digest it with the same two enzymes. You then run a gel which is shown to the right.

You mix the potato DNA (digested with the enzyme you specified in part B) with cloning vector DNA (digested with the same enzyme). You then add the mixture to E. coli cells and carry out a transformation procedure so that the cells can each update a plasmid. You then plate the cells on a plate containing antibiotic. What antibiotic would be in the plate? Why would there be antibiotic in the plate? Be specific!
Unfortunately, you don’t get a single bacterial colony to grow on the plate. Not even one! You review your procedure and realize that when mixing the digested potato DNA and digested plasmid that you forget to add a critical enzyme. What enzyme needed to be added? Why? Be very specific in your explanation.

2500
lacza
396-454
Polylinker
500
аmp
PUC19
2686 bp
2000
ori
1000
1500

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