19. You run a PCR of the above HBB sequence using the primers you designed. You receive a vectorfrom a company that you want to clone your HBB PCR product into. Below is the vector:BamHI0.5 kbEcoRI1.0 kb0.3 kb-Bglllplasmid A0.2 kbBamHI0.4 kbHindIII1.0 kbEcoRIYou want to cut this vector with BamHI and EcoRI andinsert your PCR product into this vector. After cuttingthe vector with both enzymes simultaneously you runa gel.i) On the gel below draw the bands you expect to see after you cut this vectorwith these enzymes at the same time (only use one gel lane - the other oneis going to be empty). (6 points)Ladder5kb3kb2.5kb2kb1.5kb1kb0.5kb0.3kb0.2kbii) Circle the band that represents the backbone that you will insert the PCRproduct into. (2 points)c) Once you have inserted the PCR product into the plasmid backbone from part C, howmany bp will the new vector be (the total of the backbone and the PCR producttogether). Answer should be 2 significant figures. (2 points)

i) Gel Electrophoresis Bands:- The ladder serves as a reference for the sizes of the DNA fragments.-…

Answered: 19. You run a PCR of the above HBB…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You want to set up 50 µl total volume of a PCR reaction. You have a microfuge tube of Forward primer (25 µM), and each PCR reaction requires a final of 1 uM of primer. You have a microfuge tube of Reverse primer (25 µM), and each PCR reaction requires a final of 1 µM of primer. How much of the primers would you add? Select all that apply 2 ul of Reverse primer 2 ul of Forward primer 5 ul of Forward primer 5 ul of Reverse primer
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You are studying a genome that has 42% G:C content and the remaining 58% of the genome is As and Ts. Assuming a random distribution of these bases what is the expected distance (ie there will be a cut once in every XX base pairs) between cut-sites for restriction enzyme that cuts at the sequence GAATTC? 256 3207 4168 4096
A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic regions. The insect’s genomic DNA has been treated with the restriction enzyme EcoRI. One of the fragments (with a length of 4 kb) produced in this manner is isolated and ligated into a pUC19 plasmid vector that has also been treated with EcoRI. This recombinant construct is called plasmid A. Samples of plasmid A, pUC 19, and the total genomic DNA from the insect are digested completely with the restriction enzyme EcoRI. The three digests are loaded into separate wells: plasmid A (lane 1), pUC19 (lane2) and total insect genomic DNA (lane 3) of an agarose gel and subjected to electrophoresis. 2b. Draw what will be seen in each lane if the gel is transferred to a membrane and subjected to Southern blotting and the 4000 base pair genomic DNA fragment (labeled with a fluorescent tag) from the insect is used to probe the blot.
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You receive purified mRNA that codes for the spike protein of SARS-CoV-2 as well as plasmid pET32(a+). Explain how you will prepare SARS-CoV-2 spike cDNA and name the enzymes that you will need how you will clone the cDNA into pET32(a+) and select the recombinant plasmid how you will then produce a recombinant SARS-CoV-2 spike and purify it
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A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA? a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb b. 2.0kb c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bp
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