51. In 1973, Cohen and Boyer created the first chimera by successfully incorporating the frog rRNA into bacteria. The restriction endonuclease EcoRI was used to cut the frog DNA that contained the rRNA gene and the vector plasmid pSC101. The DNA fragments and the vector plasmid pSC101 were combined and transferred to E. coli bacteria. The frog DNA fragment and pSC101 were able to combine when Select one: a. a pyrimidine base in the sticky end of the DNA fragment came in contact with a pyrimidine base in pSC101 b. a nucleotide in the sticky end of the DNA fragment came in contact with a complementary base in pSC101 c. a nucleotide in the sticky end of the DNA fragment came in contact with the same base in pSC101 d. a purine base in the sticky end of the DNA fragment came in contact with a purine base in pSC101
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
51.
The frog DNA fragment and pSC101 were able to combine when
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