A hypothetical map of a 10,000 bp (base pair) segment of a human chromosome is shown (1 kbp= 1,000 bp).
DNA is extracted from the blood cells of two different humans, individuals 1 and 2. In separate experiments, the DNA from each individual is cleaved by restriction endonucleases A, B, and C, and the fragments are separated by electrophoresis. A hypothetical map of a 10,000 bp (base pair) segment of a human chromosome is shown (1 kbp= 1,000 bp). Individual 2 has point mutations that eliminate restriction recognition sites B* and C*. After the 10kbp segment is digested with each restriction endonuclease A, B, and C one by one, samples are loaded onto an agarose gel for electrophoresis for analysis. I would like you to draw the result of gel electrophoresis. To answer the question, first, draw the whole gel image (on the right) on your answer sheet, then indicate where you expect to see the bands on the gel. (Hint1: Same-size bands will appear at the same position. Hint 2: On the gel bands from individuals 1 and 2 might be at different positions.) The left lane (with an M label on the top) of the gel are marking the size of each band in the diagram.
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