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A team of scientist is interested in the amplification of a specific DNA fragment in a large plasmid of about 10000 bps. (1) The sequence the scientists are interested in is 5-CATTGATTATTG[ 3-GTAACTAATAAG[ JATCAATTACGGG-3 JTAGTTAATGCCC-5 Where [...] indicates a longer 100bps sequence. Can you suggest two possible primers that the scientists should use to address their need, if they want to be sure they specifically address this region in the entire plasmid? ii) How can the scientist verify that their selection of primers was able to restrict the implementation to only that region of the plasmid?

A team of scientist is interested in the amplification of a specific DNA fragment in a large plasmid of about 10000 bps. (1) The sequence the scientists are interested in is 5-CATTGATTATTG[ 3-GTAACTAATAAG[ JATCAATTACGGG-3 JTAGTTAATGCCC-5 Where [...] indicates a longer 100bps sequence. Can you suggest two possible primers that the scientists should use to address their need, if they want to be sure they specifically address this region in the entire plasmid? ii) How can the scientist verify that their selection of primers was able to restrict the implementation to only that region of the plasmid?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A team of scientist is interested in the amplification of a specific DNA fragment in a large plasmid of about 10000 bps. (1) The sequence the scientists are interested in is 5-CATTGATTATTG[ 3-GTAACTAATAAG[ JATCAATTACGGG-3 JTAGTTAATGCCC-5 Where [...] indicates a longer 100bps sequence. Can you suggest two possible primers that the scientists should use to address their need, if they want to be sure they specifically address this region in the entire plasmid? ii) How can the scientist verify that their selection of primers was able to restrict the implementation to only that region of the plasmid?
Transcribed Image Text:A team of scientist is interested in the amplification of a specific DNA fragment in a large plasmid of about 10000 bps. (1) The sequence the scientists are interested in is 5-CATTGATTATTG[ 3-GTAACTAATAAG[ JATCAATTACGGG-3 JTAGTTAATGCCC-5 Where [...] indicates a longer 100bps sequence. Can you suggest two possible primers that the scientists should use to address their need, if they want to be sure they specifically address this region in the entire plasmid? ii) How can the scientist verify that their selection of primers was able to restrict the implementation to only that region of the plasmid?
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