For the following sequence design the forward and reverse primer... explain and justify your answer. Gene of Interest: a tgaaacaaca aaaacggctt tacgcccgat tgctgacgct gttatttgcg 61 ctcatcttct tgctgcctca ttctgcagca gcggcggcaa atcttaatgg gacgctgatg 121 cagtattttg aatggtacat gcccaatgac ggccaacatt ggaagcgttt gcaaaacgac 181 tcggcatatt tggctgaaca cggtattact gccgtctgga ttcccccggc atataaggga 241 acgagccaag cggatgtggg ctacggtgct tacgaccttt atgatttagg ggagtttcat 301 caaaaaggga cggttcggac aaagtacggc acaaaaggag agctgcaatc tgcgatcaaa 361 agtcttcatt cccgcgacat taacgtttac ggggatgtgg tcatcaacca caaaggcggc 421 gctgatgcga ccgaagatgt aaccgcggtt gaagtcgatc ccgctgaccg caaccgcgta 481 atttcaggag aacacctaat taaagcctgg acacattttc attttccggg gcgcggcagc 541 acatacagcg attttaaatg gcattggtac cattttgacg gaaccgattg ggacgagtcc 601 cgaaagctga accgcatcta taagtttcaa ggaaaggctt gggattggga agtttccaat 661 gaaaacggca actatgatta tttgatgtat gccgacatcg attatgacca tcctgatgtc 721 gcagcagaaa ttaagagatg gggcacttgg tatgccaatg aactgcaatt ggacggtttc 781 cgtcttgatg ctgtcaaaca cattaaattt tcttttttgc gggattgggt taatcatgtc 841 agggaaaaaa cggggaagga aatgtttacg gtagctgaat attggcagaa tgacttgggc 901 gcgctggaaa actatttgaa caaaacaaat tttaatcatt cagtgtttga cgtgccgctt 961 cattatcagt tccatgctgc atcgacacag ggaggcggct atgatatgag gaaattgctg 1021 aacggtacgg tcgtttccaa gcatccgttg aaatcggtta catttgtcga taaccatgat 1081 acacagccgg ggcaatcgct tgagtcgact gtccaaacat ggtttaagcc gcttgcttac 1141 gcttttattc tcacaaggga atctggatac cctcaggttt tctacgggga tatgtacggg 1201 acgaaaggag actcccagcg cgaaattcct gccttgaaac acaaaattga accgatctta 1261 aaagcgagaa aacagtatgc gtacggagca cagcatgatt atttcgacca ccatgacatt 1321 gtcggctgga caagggaagg cgacagctcg gttgcaaatt caggtttggc ggcattaata 1381 acagacggac ccggtggggc aaagcgaatg tatgtcggcc ggcaaaacgc cggtgagaca 1441 tggcatgaca ttaccggaaa ccgttcggag ccggttgtca tcaattcgga aggctgggga 1501 gagtttcacg taaacggcgg gtcggtttca atttatgttc aaagatag
For the following sequence design the forward and reverse primer... explain and justify your answer.
Gene of Interest:
a tgaaacaaca aaaacggctt tacgcccgat tgctgacgct gttatttgcg 61 ctcatcttct tgctgcctca ttctgcagca gcggcggcaa atcttaatgg gacgctgatg 121 cagtattttg aatggtacat gcccaatgac ggccaacatt ggaagcgttt gcaaaacgac 181 tcggcatatt tggctgaaca cggtattact gccgtctgga ttcccccggc atataaggga 241 acgagccaag cggatgtggg ctacggtgct tacgaccttt atgatttagg ggagtttcat 301 caaaaaggga cggttcggac aaagtacggc acaaaaggag agctgcaatc tgcgatcaaa 361 agtcttcatt cccgcgacat taacgtttac ggggatgtgg tcatcaacca caaaggcggc 421 gctgatgcga ccgaagatgt aaccgcggtt gaagtcgatc ccgctgaccg caaccgcgta 481 atttcaggag aacacctaat taaagcctgg acacattttc attttccggg gcgcggcagc 541 acatacagcg attttaaatg gcattggtac cattttgacg gaaccgattg ggacgagtcc 601 cgaaagctga accgcatcta taagtttcaa ggaaaggctt gggattggga agtttccaat 661 gaaaacggca actatgatta tttgatgtat gccgacatcg attatgacca tcctgatgtc 721 gcagcagaaa ttaagagatg gggcacttgg tatgccaatg aactgcaatt ggacggtttc 781 cgtcttgatg ctgtcaaaca cattaaattt tcttttttgc gggattgggt taatcatgtc 841 agggaaaaaa cggggaagga aatgtttacg gtagctgaat attggcagaa tgacttgggc 901 gcgctggaaa actatttgaa caaaacaaat tttaatcatt cagtgtttga cgtgccgctt 961 cattatcagt tccatgctgc atcgacacag ggaggcggct atgatatgag gaaattgctg 1021 aacggtacgg tcgtttccaa gcatccgttg aaatcggtta catttgtcga taaccatgat 1081 acacagccgg ggcaatcgct tgagtcgact gtccaaacat ggtttaagcc gcttgcttac 1141 gcttttattc tcacaaggga atctggatac cctcaggttt tctacgggga tatgtacggg 1201 acgaaaggag actcccagcg cgaaattcct gccttgaaac acaaaattga accgatctta 1261 aaagcgagaa aacagtatgc gtacggagca cagcatgatt atttcgacca ccatgacatt 1321 gtcggctgga caagggaagg cgacagctcg gttgcaaatt caggtttggc ggcattaata 1381 acagacggac ccggtggggc aaagcgaatg tatgtcggcc ggcaaaacgc cggtgagaca 1441 tggcatgaca ttaccggaaa ccgttcggag ccggttgtca tcaattcgga aggctgggga 1501 gagtttcacg taaacggcgg gtcggtttca atttatgttc aaagatag
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