115919 5'-TGTAAACCggAgAt 160271 GAAAACCtttTtCCAAGGCAaaTCAGAGAAAAAGTCTTTAACTCCACCATTAGCACCCаAAGCTAAGATTCTAATTTAAACTALTCTCTGTTCT-3' 3'-ACATTCGGCCTcTaCTTTTGGaaaAaGgTTCCtGTttAGTCTCTTTTTCAGAAATTGAGGTGGTAATCGTGGGTTCGATTCTAAGATTAAATTTGATAAGAGACAAGA-5' 11 116028 Forward primer binding region 1 T | | 1 | | 1 | 109 16136| 5'-TTCATGGGGaagCagATTTGgGtacCACCCAagtAtTGacTcaccCatcAAcaaccgctATgTAtttcGta CATtActgccagccaccAtgaaTattGtaCagtaccat-3' 3'-AAGTACCCCttcGtcTAAACCCatgGTGGGTtcaTaACtgAgtggGtagTTgttggcgaTACATaaagCatGTAaTgacggtcgGtggTacttAtaa | 110 | | | | CatGtcatggta-5' | 2181 116137 16245 5'-aaatAcTtgaccAcCtgtagtaCataaaaacccaatccacatcaaaaccctccccccatgctTAcAagcaagtacagcaatcaaccttcAactgtcacacaTcaaCtgc-3' 3'-tttaTgAactggTgGacatcatGtatttttgggttaggtgtagttttgggaggggggtacgaATgTtcgttcatgtcgttagttggaagTtgacagtgtgtAgttGacg-5' 1219 | 1 | 3271 116246 | 163541 5'-aactcCaaagccacccctcacCcactaggaTatcaaCAaacctacccacccttaacagtacAtagcaca TaaagccatttaccGtacatagcacaTtacagtcaaatcc-3' 1328 | 3'-ttgagGtttcggtggggagtgGgtgatcctAtagtt GTttggatgggtgggaattgtcatgTatcgtgtAtttcggtaaatggCatgtatcgtgtAatgtcagtttagg-5' | | | 4361 | | | 16463| 116355 | | | 5'-cttctcgtccccatGGaTGACCCCCctCAgATAgggg TCCCTTgacCACCATCCTCCGTGAAAtcAAtAtCCCgcACAAGAGTGCACTCTCCTCGCTCCGgGCCCAT-3' 3'-gaagagcaggggtaCCtACTgGgGGgaGTCTATccccAGGgAActgGTGGTAGGAGGCACTTTAgTTaTaGGGcgTGTTCTCACGATGAGAGGAGCGAGGCCCGGGTAt-5' 1437 116464 | I | Reverse primer binding region | 5451 1165691 5'-ACACtTggGgGTAGCTAAagTGAACTGTATCCGACACTGGTTCCTACTTCAGGGCCATAaAgcCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATG-3'-end 3'-TgTGaAccCcCATCGATTtCACTTgACATAGGCtGTAGACCAAGGATGAAGTCCcgGTATtTcgGATTTATCGGGTGTGCAAGGGGAATTTATTCTGTAGTGCTAC-5'-end 1546 | 6511
115919 5'-TGTAAACCggAgAt 160271 GAAAACCtttTtCCAAGGCAaaTCAGAGAAAAAGTCTTTAACTCCACCATTAGCACCCаAAGCTAAGATTCTAATTTAAACTALTCTCTGTTCT-3' 3'-ACATTCGGCCTcTaCTTTTGGaaaAaGgTTCCtGTttAGTCTCTTTTTCAGAAATTGAGGTGGTAATCGTGGGTTCGATTCTAAGATTAAATTTGATAAGAGACAAGA-5' 11 116028 Forward primer binding region 1 T | | 1 | | 1 | 109 16136| 5'-TTCATGGGGaagCagATTTGgGtacCACCCAagtAtTGacTcaccCatcAAcaaccgctATgTAtttcGta CATtActgccagccaccAtgaaTattGtaCagtaccat-3' 3'-AAGTACCCCttcGtcTAAACCCatgGTGGGTtcaTaACtgAgtggGtagTTgttggcgaTACATaaagCatGTAaTgacggtcgGtggTacttAtaa | 110 | | | | CatGtcatggta-5' | 2181 116137 16245 5'-aaatAcTtgaccAcCtgtagtaCataaaaacccaatccacatcaaaaccctccccccatgctTAcAagcaagtacagcaatcaaccttcAactgtcacacaTcaaCtgc-3' 3'-tttaTgAactggTgGacatcatGtatttttgggttaggtgtagttttgggaggggggtacgaATgTtcgttcatgtcgttagttggaagTtgacagtgtgtAgttGacg-5' 1219 | 1 | 3271 116246 | 163541 5'-aactcCaaagccacccctcacCcactaggaTatcaaCAaacctacccacccttaacagtacAtagcaca TaaagccatttaccGtacatagcacaTtacagtcaaatcc-3' 1328 | 3'-ttgagGtttcggtggggagtgGgtgatcctAtagtt GTttggatgggtgggaattgtcatgTatcgtgtAtttcggtaaatggCatgtatcgtgtAatgtcagtttagg-5' | | | 4361 | | | 16463| 116355 | | | 5'-cttctcgtccccatGGaTGACCCCCctCAgATAgggg TCCCTTgacCACCATCCTCCGTGAAAtcAAtAtCCCgcACAAGAGTGCACTCTCCTCGCTCCGgGCCCAT-3' 3'-gaagagcaggggtaCCtACTgGgGGgaGTCTATccccAGGgAActgGTGGTAGGAGGCACTTTAgTTaTaGGGcgTGTTCTCACGATGAGAGGAGCGAGGCCCGGGTAt-5' 1437 116464 | I | Reverse primer binding region | 5451 1165691 5'-ACACtTggGgGTAGCTAAagTGAACTGTATCCGACACTGGTTCCTACTTCAGGGCCATAaAgcCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATG-3'-end 3'-TgTGaAccCcCATCGATTtCACTTgACATAGGCtGTAGACCAAGGATGAAGTCCcgGTATtTcgGATTTATCGGGTGTGCAAGGGGAATTTATTCTGTAGTGCTAC-5'-end 1546 | 6511
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
How would you write out the sequence for the REVERSE primer if you needed to purchase it for use in PCR?
ATTTCGGATTTATCGGGTGT
GTTATGAACCCACCATGGGT
TGTGGGCTATTTAGGCTTTA
ACCCATGGTGGGTTCATAAC
TAAAGCCTAAATAGCCCACA
TGGGTACCACCCAAGTATTG
Transcribed Image Text:115919
5'-TGTAAACCggAgAt
160271
GAAAACCtttTtCCAAGGCAaaTCAGAGAAAAAGTCTTTAACTCCACCATTAGCACCCаAAGCTAAGATTCTAATTTAAACTALTCTCTGTTCT-3'
3'-ACATTCGGCCTcTaCTTTTGGaaaAaGgTTCCtGTttAGTCTCTTTTTCAGAAATTGAGGTGGTAATCGTGGGTTCGATTCTAAGATTAAATTTGATAAGAGACAAGA-5'
11
116028
Forward primer binding region
1
T
|
|
1
|
|
1
|
109
16136|
5'-TTCATGGGGaagCagATTTGgGtacCACCCAagtAtTGacTcaccCatcAAcaaccgctATgTAtttcGta CATtActgccagccaccAtgaaTattGtaCagtaccat-3'
3'-AAGTACCCCttcGtcTAAACCCatgGTGGGTtcaTaACtgAgtggGtagTTgttggcgaTACATaaagCatGTAaTgacggtcgGtggTacttAtaa
| 110
|
|
|
|
CatGtcatggta-5'
| 2181
116137
16245
5'-aaatAcTtgaccAcCtgtagtaCataaaaacccaatccacatcaaaaccctccccccatgctTAcAagcaagtacagcaatcaaccttcAactgtcacacaTcaaCtgc-3'
3'-tttaTgAactggTgGacatcatGtatttttgggttaggtgtagttttgggaggggggtacgaATgTtcgttcatgtcgttagttggaagTtgacagtgtgtAgttGacg-5'
1219
|
1
| 3271
116246
|
163541
5'-aactcCaaagccacccctcacCcactaggaTatcaaCAaacctacccacccttaacagtacAtagcaca TaaagccatttaccGtacatagcacaTtacagtcaaatcc-3'
1328
|
3'-ttgagGtttcggtggggagtgGgtgatcctAtagtt GTttggatgggtgggaattgtcatgTatcgtgtAtttcggtaaatggCatgtatcgtgtAatgtcagtttagg-5'
|
|
|
4361
|
|
|
16463|
116355 |
|
|
5'-cttctcgtccccatGGaTGACCCCCctCAgATAgggg TCCCTTgacCACCATCCTCCGTGAAAtcAAtAtCCCgcACAAGAGTGCACTCTCCTCGCTCCGgGCCCAT-3'
3'-gaagagcaggggtaCCtACTgGgGGgaGTCTATccccAGGgAActgGTGGTAGGAGGCACTTTAgTTaTaGGGcgTGTTCTCACGATGAGAGGAGCGAGGCCCGGGTAt-5'
1437
116464
|
I
|
Reverse primer binding region
|
5451
1165691
5'-ACACtTggGgGTAGCTAAagTGAACTGTATCCGACACTGGTTCCTACTTCAGGGCCATAaAgcCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATG-3'-end
3'-TgTGaAccCcCATCGATTtCACTTgACATAGGCtGTAGACCAAGGATGAAGTCCcgGTATtTcgGATTTATCGGGTGTGCAAGGGGAATTTATTCTGTAGTGCTAC-5'-end
1546
| 6511
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