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5'-GATTACAATAGGAATTC~~ GAATTCTCGTGACTCCG-3' 3'-CTAATGTTATCCTTAAG~ CTTAAGAGCACTGAGGC-5' Which of the following pairs of primers would amplify this fragment? (Hint: Sequence and orientation are re considerations). O None of these sequences O 5'-GATTACA-3' and 5'-GCCTCAG-3' O 5'-GATTACA-3' and 5'-CGGAGTC-3' O 5'-CTAATGT-3' and 5'-CGGAGTC-3' O 5'-CTAATGT-3' and 5'-GCCTCAG-3'

5'-GATTACAATAGGAATTC~~ GAATTCTCGTGACTCCG-3' 3'-CTAATGTTATCCTTAAG~ CTTAAGAGCACTGAGGC-5' Which of the following pairs of primers would amplify this fragment? (Hint: Sequence and orientation are re considerations). O None of these sequences O 5'-GATTACA-3' and 5'-GCCTCAG-3' O 5'-GATTACA-3' and 5'-CGGAGTC-3' O 5'-CTAATGT-3' and 5'-CGGAGTC-3' O 5'-CTAATGT-3' and 5'-GCCTCAG-3'

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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### Amplifying a DNA Fragment Using PCR #### Objective: You want to amplify a DNA fragment that has the terminal sequences shown below: 5’-GATTACAATAGGAATTC~~~~~~~~~~~~~~~~GAATTCTCGTGACTCCG-3’ 3’-CTAATGTTATCCTTAAG~~~~~~~~~~~~~~~CTTAAGAGCACTGAGGC-5’ #### Question: Which of the following pairs of primers would amplify this fragment? (Hint: Sequence and orientation are relevant considerations). ##### Options: 1. None of these sequences 2. 5’-GATTACA-3’ and 5’-GCCTCAG-3’ 3. 5’-GATTACA-3’ and 5’-CGGAGTC-3’ 4. 5’-CTAATGT-3’ and 5’-CGGAGTC-3’ 5. 5’-CTAATGT-3’ and 5’-GCCTCAG-3’ ##### Explanation: To determine the correct pair of primers for amplifying the given DNA sequence, recall that primers need to be complementary to the 3’ ends of the template DNA strand. This ensures that the DNA polymerase can add nucleotides to the new strand, synthesizing in the 5' to 3' direction. For the 5’ to 3’ strand (top strand), the primer should be complementary to the start of the sequence, and for the 3’ to 5’ strand (bottom strand), the primer should be complementary to the end of the sequence: - For the top strand (5’-GATTACAATAGGAATTC): - Potential primer: 5’-GATTACA-3’ (since it matches from 5’ end) - For the bottom strand (3’-CTTAAGAGCACTGAGGC): - Potential primer: 5’-GCCTCAG-3’ (since it matches complementary 3’ end) Thus, the correct answer would be **Option 2: 5’-GATTACA-3’ and 5’-GCCTCAG-3’**
Transcribed Image Text:### Amplifying a DNA Fragment Using PCR #### Objective: You want to amplify a DNA fragment that has the terminal sequences shown below: 5’-GATTACAATAGGAATTC~~~~~~~~~~~~~~~~GAATTCTCGTGACTCCG-3’ 3’-CTAATGTTATCCTTAAG~~~~~~~~~~~~~~~CTTAAGAGCACTGAGGC-5’ #### Question: Which of the following pairs of primers would amplify this fragment? (Hint: Sequence and orientation are relevant considerations). ##### Options: 1. None of these sequences 2. 5’-GATTACA-3’ and 5’-GCCTCAG-3’ 3. 5’-GATTACA-3’ and 5’-CGGAGTC-3’ 4. 5’-CTAATGT-3’ and 5’-CGGAGTC-3’ 5. 5’-CTAATGT-3’ and 5’-GCCTCAG-3’ ##### Explanation: To determine the correct pair of primers for amplifying the given DNA sequence, recall that primers need to be complementary to the 3’ ends of the template DNA strand. This ensures that the DNA polymerase can add nucleotides to the new strand, synthesizing in the 5' to 3' direction. For the 5’ to 3’ strand (top strand), the primer should be complementary to the start of the sequence, and for the 3’ to 5’ strand (bottom strand), the primer should be complementary to the end of the sequence: - For the top strand (5’-GATTACAATAGGAATTC): - Potential primer: 5’-GATTACA-3’ (since it matches from 5’ end) - For the bottom strand (3’-CTTAAGAGCACTGAGGC): - Potential primer: 5’-GCCTCAG-3’ (since it matches complementary 3’ end) Thus, the correct answer would be **Option 2: 5’-GATTACA-3’ and 5’-GCCTCAG-3’**
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