10050104110104CotDNA renaturation curves occasionally show three distinct phases ofrenaturation. In this plot, DNA renaturation is plotted against “C,t"(initial concentration times time of renaturation, essentially a meas-ure of relative renaturation time).(a) Identify each part of this plot that corresponds to reannealing of(1) unique sequences, (2) moderately repetitive sequences, and(3) highly repetitive sequences.(b) Suppose that you cloned a single-copy gene, such as the gene fordihydrofolate reductase (DHFR), and subjected this to renaturationanalysis. Sketch the curve you might expect.(c) Suppose that you cloned the DHFR CDNA instead of genomicDNA. Would you expect this to reanneal (1) more slowly, (2) morerapidly, or (3) at the same rate as genomic DNA? Briefly explain youranswer.% Single stranded

Answered: 100 50 104 1 10 104 Cot DNA…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Related questions

Question

100
50
104
1
10
104
Cot
DNA renaturation curves occasionally show three distinct phases of
renaturation. In this plot, DNA renaturation is plotted against “C,t"
(initial concentration times time of renaturation, essentially a meas-
ure of relative renaturation time).
(a) Identify each part of this plot that corresponds to reannealing of
(1) unique sequences, (2) moderately repetitive sequences, and
(3) highly repetitive sequences.
(b) Suppose that you cloned a single-copy gene, such as the gene for
dihydrofolate reductase (DHFR), and subjected this to renaturation
analysis. Sketch the curve you might expect.
(c) Suppose that you cloned the DHFR CDNA instead of genomic
DNA. Would you expect this to reanneal (1) more slowly, (2) more
rapidly, or (3) at the same rate as genomic DNA? Briefly explain your
answer.
% Single stranded

Expert Solution

Step 1

A single stranded DNA binds to its complementary strand to reform double stranded DNA is called renaturation.

Common sequences renature more quickly than rare sequences.

The rate at which a sequence will re-associate is directly proportional to the number of copies of that sequence in the DNA sample.

The amount of renaturation is measured relative to a C₀t value. The C₀t value is the product of C₀ (the initial concentration of DNA), t (time in seconds), and a constant that depends on the concentration of cations in the buffer.

Repetitive DNA renature at low C₀t values, whereas complex and unique DNA sequences renature at high C₀t values. The quick renaturation of the repetitive DNA is because of the presence of numerous complementary sequences.

Step 2