How do I fill in each column for each DNA fragment?

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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How do I fill in each column for each DNA fragment?
**Figure 11-2: Professional gel of Lambda digest by HindIII, EcoRI, and BamHI**

This image depicts an electrophoresis gel used for analyzing DNA fragments from a Lambda phage treated with three different restriction enzymes: HindIII, EcoRI, and BamHI. The gel also includes uncut Lambda DNA for comparison.

**Description of Gel Lanes:**

1. **BamHI lane:** 
   - Displays several distinct bands, indicating that the BamHI enzyme cuts the Lambda DNA into multiple fragments of varying sizes.

2. **EcoRI lane:** 
   - Similar to the BamHI lane, this shows multiple bands, which represent the different-sized fragments produced by EcoRI digestion.

3. **HindIII lane:** 
   - Features a series of bands that correspond to the specific fragments generated by HindIII's restriction activity on Lambda DNA.

4. **Uncut lambda lane:** 
   - Shows a single, high molecular weight band, representing the intact Lambda DNA molecule.

**Side Ruler:**
- A ruler is visible on the left side, labeled with numbers likely representing molecular weight markers to determine the size of DNA fragments.

This gel is used to evaluate the effectiveness and results of DNA digestion by specific restriction enzymes, important for various genetic and molecular biology applications.
Transcribed Image Text:**Figure 11-2: Professional gel of Lambda digest by HindIII, EcoRI, and BamHI** This image depicts an electrophoresis gel used for analyzing DNA fragments from a Lambda phage treated with three different restriction enzymes: HindIII, EcoRI, and BamHI. The gel also includes uncut Lambda DNA for comparison. **Description of Gel Lanes:** 1. **BamHI lane:** - Displays several distinct bands, indicating that the BamHI enzyme cuts the Lambda DNA into multiple fragments of varying sizes. 2. **EcoRI lane:** - Similar to the BamHI lane, this shows multiple bands, which represent the different-sized fragments produced by EcoRI digestion. 3. **HindIII lane:** - Features a series of bands that correspond to the specific fragments generated by HindIII's restriction activity on Lambda DNA. 4. **Uncut lambda lane:** - Shows a single, high molecular weight band, representing the intact Lambda DNA molecule. **Side Ruler:** - A ruler is visible on the left side, labeled with numbers likely representing molecular weight markers to determine the size of DNA fragments. This gel is used to evaluate the effectiveness and results of DNA digestion by specific restriction enzymes, important for various genetic and molecular biology applications.
**Activity 2 – Paper Exercise from a Professional Gel**

**Table 11-2: Professional Gel – Relationship of Movement of DNA to Fragment Size**

This table provides data on DNA fragment sizes generated by different restriction enzymes and outlines the relationship between the movement of these fragments on a gel and their sizes.

| **HindIII (known)** |               | **EcoRI**          |               | **BamHI**          |               |
|---------------------|---------------|--------------------|---------------|--------------------|---------------|
| **Distance**        | **Actual size in bp** | **Distance**  | **Estimated size in bp** | **Distance**  | **Estimated size in bp** |
|                     | 23130         |                    |               |                    |               |
|                     | 9416          |                    |               |                    |               |
|                     | 6557          |                    |               |                    |               |
|                     | 4361          |                    |               |                    |               |
|                     | 2322          |                    |               |                    |               |
|                     | 2027          |                    |               |                    |               |
|                     | 564           |                    |               |                    |               |
|                     | 125           |                    |               |                    |               |

**Attachments:**

1. Professional gel and standard curve on log paper (p 57-58)

**Explanation:**

This table documents known sizes of DNA fragments when digested with the HindIII enzyme, measured in base pairs (bp). Similar columns for EcoRI and BamHI are included for recording distances and estimated fragment sizes, which are to be determined.

Using a standard curve, the distances traveled by DNA fragments can be plotted to estimate their sizes for EcoRI and BamHI, based on the known standards provided by HindIII. This exercise helps understand how DNA moves through a gel matrix during electrophoresis and illustrates the principle of size estimation using a reference (standard curve).
Transcribed Image Text:**Activity 2 – Paper Exercise from a Professional Gel** **Table 11-2: Professional Gel – Relationship of Movement of DNA to Fragment Size** This table provides data on DNA fragment sizes generated by different restriction enzymes and outlines the relationship between the movement of these fragments on a gel and their sizes. | **HindIII (known)** | | **EcoRI** | | **BamHI** | | |---------------------|---------------|--------------------|---------------|--------------------|---------------| | **Distance** | **Actual size in bp** | **Distance** | **Estimated size in bp** | **Distance** | **Estimated size in bp** | | | 23130 | | | | | | | 9416 | | | | | | | 6557 | | | | | | | 4361 | | | | | | | 2322 | | | | | | | 2027 | | | | | | | 564 | | | | | | | 125 | | | | | **Attachments:** 1. Professional gel and standard curve on log paper (p 57-58) **Explanation:** This table documents known sizes of DNA fragments when digested with the HindIII enzyme, measured in base pairs (bp). Similar columns for EcoRI and BamHI are included for recording distances and estimated fragment sizes, which are to be determined. Using a standard curve, the distances traveled by DNA fragments can be plotted to estimate their sizes for EcoRI and BamHI, based on the known standards provided by HindIII. This exercise helps understand how DNA moves through a gel matrix during electrophoresis and illustrates the principle of size estimation using a reference (standard curve).
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