100501011010CotDNA renaturation curves occasionally show three distinct phases of rena-turation. In this graph, DNA renaturation is plotted against "Cf" (initialconcentration times time of renaturation-essentially a measure of relativerenaturation time). See Figure 21.4.(a) Identify each part of this plot that corresponds to reannealing of(1) unique sequences, (2) moderately repetitive sequences, and (3) highlyrepetitive sequences.(b) Suppose that you cloned a single-copy gene, such as the gene for dihy-drofolate reductase (DHFR), into a plasmid vector and subjected it torenaturation analysis. Sketch the curve you might expect.(c) Suppose that you used reverse transcriptase (Chapter 24) to copy theovalbumin mRNA and cloned this complementary DNA (CDNA) intoa plasmid vector. Would you expect this CDNA to reanneal (1) moreslowly. (2) more rapidly, or (3) at the same rate as genomic DNA? Brieflyexplain your answer.% Single stranded

The denatured DNA can reformulate hydrogen bonds between complementary single strand, making it…

Answered: 100 50 10 1 10 10 Cot DNA renaturation…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Can you please help with 1f please picture with 1 graph is for question 1a) picture with 4 graphs is for question 1b) 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation, you will find that all the DNA bands at the same place in the gradient, and if you graph the distribution of DNA fragments in the gradient you will get a single peak (see below). If you perform the same experiment with binturong DNA, you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to a small "satellite" peak on a graph of the distribution of DNA fragments in the gradient (see below). Why do these two DNA samples give different results, when they're both 50% G-C? 1b) If you denatured the random 1000 bp fragments of binturong DNA that you produced in question 1a by heating them to 95ºC, and then cooled them down to 60ºC and…
Can you please help with 1c please picture with 1 graph is for question 1a) picture with 4 graphs is for question 1b) 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation, you will find that all the DNA bands at the same place in the gradient, and if you graph the distribution of DNA fragments in the gradient you will get a single peak (see below). If you perform the same experiment with binturong DNA, you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to a small "satellite" peak on a graph of the distribution of DNA fragments in the gradient (see below). Why do these two DNA samples give different results, when they're both 50% G-C? 1b) If you denatured the random 1000 bp fragments of binturong DNA that you produced in question 1a by heating them to 95ºC, and then cooled them down to 60ºC…
Please explain in detail....
Can you please help with 1c please picture with 1 graph is for question 1a) picture with 4 graphs is for question 1b) 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation, you will find that all the DNA bands at the same place in the gradient, and if you graph the distribution of DNA fragments in the gradient you will get a single peak (see below). If you perform the same experiment with binturong DNA, you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to a small "satellite" peak on a graph of the distribution of DNA fragments in the gradient (see below). Why do these two DNA samples give different results, when they're both 50% G-C? 1b) If you denatured the random 1000 bp fragments of binturong DNA that you produced in question 1a by heating them to 95ºC, and then cooled them down to 60ºC…
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What would be the anticipated caloric energy needed to construct the human genome from scratch (approximately 3 billion base pairs on 23 individual chromosomes in a diploid state)? Assume that all the purine and pyrimidine bases are pre-existing and abundant ATP and replicating enzymes are not limiting. What would be the estimated daily energy requirement to maintain nuclear supercoiled DNA available for synthetic activities and not quiescent?
Please in detail, what the picture attached represents
18 Fred Sanger developed an efective DNA sequencing technique in 1977. The peed at which DNA.can be sequenced has been increasing rapidly since the introduction of DNA sequencing The length of DNA that can be sequenced in a given time is measured in base pairs or kilobase pairs. In 1980, the speed at which DNA could be sequenced by a single machine was approximately 500 base palrs per hour. In 2016 that speed had increased to approximately 50 million kilobase pairs per hour. Calculate how many times faster the speed of DNA sequencing is in 2016 compared with 1980.
1) Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing, with reference to a published protocol from a company that supplies regents and kits for DNA extraction (e.g Qiagen, ThermoFischer, Invitrogen, or similar). Please Include the weblink for the published protocol you used as a reference. (Word limits: 300) 2) Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher temperatures. (Word Limits: 100)
The nucleosome assembly factor exclusively functions in DNA-dependent nucleosome assembly and binds to the PCNA (clamp) of DNA polymerase. (Capitalization and dashes/spaces are not critical.)
All are correct about DNA gyrase in E. coli EXCEPT: It works to remove positive supercoiling introduced by the DnaB protein (helicase). It is a topoisomerase that hydrolyzes ATP during its reaction mechanism. Its mechanism involves the breaking of a single phosphoester bond in one strand of dsDNA. It works to relieve supercoiling in DNA to overcome the torsion stress imposed upon unwinding.
Compute the PERCENT IDENTITY for the following pairwise sequence alignment. ACTGATGGGGG--AGACGTA ||||| ... I ||||||| ACTG--AAAAGCTAGACGTA

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