100 50 10 1 10 10 Cot DNA renaturation curves occasionally show three distinct phases of rena- turation. In this graph, DNA renaturation is plotted against "Cf" (initial concentration times time of renaturation-essentially a measure of relative renaturation time). See Figure 21.4. (a) Identify each part of this plot that corresponds to reannealing of (1) unique sequences, (2) moderately repetitive sequences, and (3) highly repetitive sequences. (b) Suppose that you cloned a single-copy gene, such as the gene for dihy- drofolate reductase (DHFR), into a plasmid vector and subjected it to renaturation analysis. Sketch the curve you might expect. (c) Suppose that you used reverse transcriptase (Chapter 24) to copy the ovalbumin mRNA and cloned this complementary DNA (CDNA) into a plasmid vector. Would you expect this CDNA to reanneal (1) more slowly. (2) more rapidly, or (3) at the same rate as genomic DNA? Briefly explain your answer. % Single stranded
100 50 10 1 10 10 Cot DNA renaturation curves occasionally show three distinct phases of rena- turation. In this graph, DNA renaturation is plotted against "Cf" (initial concentration times time of renaturation-essentially a measure of relative renaturation time). See Figure 21.4. (a) Identify each part of this plot that corresponds to reannealing of (1) unique sequences, (2) moderately repetitive sequences, and (3) highly repetitive sequences. (b) Suppose that you cloned a single-copy gene, such as the gene for dihy- drofolate reductase (DHFR), into a plasmid vector and subjected it to renaturation analysis. Sketch the curve you might expect. (c) Suppose that you used reverse transcriptase (Chapter 24) to copy the ovalbumin mRNA and cloned this complementary DNA (CDNA) into a plasmid vector. Would you expect this CDNA to reanneal (1) more slowly. (2) more rapidly, or (3) at the same rate as genomic DNA? Briefly explain your answer. % Single stranded
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:100
50
10
1
10
10
Cot
DNA renaturation curves occasionally show three distinct phases of rena-
turation. In this graph, DNA renaturation is plotted against "Cf" (initial
concentration times time of renaturation-essentially a measure of relative
renaturation time). See Figure 21.4.
(a) Identify each part of this plot that corresponds to reannealing of
(1) unique sequences, (2) moderately repetitive sequences, and (3) highly
repetitive sequences.
(b) Suppose that you cloned a single-copy gene, such as the gene for dihy-
drofolate reductase (DHFR), into a plasmid vector and subjected it to
renaturation analysis. Sketch the curve you might expect.
(c) Suppose that you used reverse transcriptase (Chapter 24) to copy the
ovalbumin mRNA and cloned this complementary DNA (CDNA) into
a plasmid vector. Would you expect this CDNA to reanneal (1) more
slowly. (2) more rapidly, or (3) at the same rate as genomic DNA? Briefly
explain your answer.
% Single stranded
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