What happened to the DNA at the different temperatures? How does Polymerase Chain Reaction exploit this property of DNA

Q: Briefly explain the rationales of adding Bisacridine A which can affect DNA stability in polymerase…

A: PCR is used to enhance genes associated with patients' DNA genetic disorders. It has practical uses…

Q: The team would now like to establish the smallest possible deletion that would inactivate the…

A: Amino acid monomers form the building blocks of proteins. The arrangement of amino acids makes up a…

Q: Explain Shortly. I need help   The emergence of new molecular biology techniques has allowed…

A: You have asked three parts of a question. Third part is a personal opinion on this topic, so I won't…

Q: 1). DNA polymerase III is the bacterial replicative polymerase. It is comprised of subunits derived…

A: DNA polymerase III transcribes the genetic code in bacteria. Polθ and Poleε subunits function as…

Q: Coomassie dye is the equivalent of what component used in analysis of DNA by agarose gel…

A: Western blot helps to identify specific proteins from a mixture of proteins and this step follows…

Q: What variables can affect the transformation efficiency?

A: Introduction  The ability of cells to absorb extracellular DNA and express the genes that are…

Q: 1. Why do we need to check the isolated DNA for its quantity and quality? 2. The purity of DNA…

A: Deoxyribonucleic acid DNA isolation is referred to the extraction process of DNA from different…

Q: The original DNA sequence was CCGGAATT. It was replicated. Which of these newly replicated DNA…

A: DNA is deoxy ribo nucleic acid which copies itself by a process called replication. This helps in…

Q: 67. To make an insertion using CRISPR Cas9, one must include a DNA with flanking sequences similar…

A:

Q: 1)explain why it is far less important for RNA Polymerase to have proofreading activity than it is…

A: Fidelity of replication is the most important for the very existence of an organism. Besides its…

Q: What is the formula for transformation efficiency?

A: Transformation: Transformation is a process of delivering a genetic element inside a bacterial cell.…

Q: Read and explain the DNA sequence below A Chromatograms from Contig[0001] MC2-wiregent base #543…

A: The image shows a chromatogram, which is a visual representation of DNA sequence data typically…

Q: Questions are talking about amplification of DNA When we run the gel, we also run a ladder why?

A: A DNA ladder is the mixture of amplified DNA fragments of different size range which is used as…

Q: The chromatogram shows fluorescent peak data from a dye-terminating nucleotide-sequencing reaction.…

A: In 1977, Frederick Sanger and his colleagues developed a method for sequencing DNA, which was later…

Q: explain why it is far less important for Primase to have proofreading activity than it is for DNA…

A: DNA proofreading is error correction process, in which DNA polymerase checks the each nucleotide…

Q: Answers to the following questions. A. You are given a tube containing 275 ng of purified PCR…

A: 5) A. Amount of purified PCR product DNA in the tube = 275 ng = 275 000 pg (1000 pg = 1 ng) The…

Q: Could you please explain these:   1. Why analyze the unknowns in a (short) serial dilution rather…

A: Introduction: DNA concentration refers to the amount of DNA present in a sample. It is typically…

Q: Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing,…

A: Although the techniques used are same, there is difference in the chemicals used by ThermoFischer.…

Q: What would spread (fragmented) bands in the electrophoresis gel tell you about the quality of your…

A: Gel electrophoresis is a technique used to separate DNA fragments according to their size. The DNA…

Q: Assume complete digestion of a single linear piece of DNA digested (cut) with EcoRI and Hindill.…

A: A plasmid is a single-stranded, circular DNA molecule that is different from the chromosomal DNA of…

Q: Which two restriction enzymes could be used to cut this DNA sequence?…

A: Restriction enzymes are proteins that recognize specific DNA sequences and cut the DNA at or near…

Q: A geneticist is interested in determining the locations of methylated cytosines within a fragment of…

A: Sodium bisulfite (NaHSO3) is a potent deamination agent. It removes an amino group (-NH2) from…

Q: Hhal is a 4-cutter restriction enzyme that creates 2-base, 3' overhangs ("sticky ends") and…

A: Restriction enzymes can cleave the DNA sequences at the recognition sites, also known as restriction…

Q: DNA ladder Sample digested with EcoRI negative electrode 23- This is the result of gel…

A: Introduction Gel electrophoresis is a technique that uses size and charge to separate and analyse…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.