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Ladder LS KT MV DY JY Ladder 850 bp 550 bp 11 1

Ladder LS KT MV DY JY Ladder 850 bp 550 bp 11 1

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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For each individual on each gel, determine if the individual is homozygous for presence of the Alu insertion(++), heterozygous (+-), homozygous for the absence of the Alu insertion (-), or if the results are inconclusive (I) (explain what makes you decide your determination, in detail). Explain what may have caused any unexpected bands (neither 850 nor 550) or lack of bands.
The image shows a table with three columns labeled "Individual," "Results," and "Explanation." The rows under "Individual" are filled with the following initials: - LS - KT - MV - DY - JY The "Results" and "Explanation" columns are currently empty for all entries. There are no graphs or diagrams present in the image.
Transcribed Image Text:The image shows a table with three columns labeled "Individual," "Results," and "Explanation." The rows under "Individual" are filled with the following initials: - LS - KT - MV - DY - JY The "Results" and "Explanation" columns are currently empty for all entries. There are no graphs or diagrams present in the image.
This image displays a gel electrophoresis result, commonly used for DNA analysis. ### Description of the Gel: - **Lanes:** - The first and last lanes (labeled "Ladder") contain a DNA ladder. This ladder serves as a reference for estimating the size of DNA fragments. Bands are visible at different positions corresponding to known base pair (bp) lengths. - Lanes labeled "LS," "KT," "MV," "DY," and "JY" contain DNA samples being analyzed, each showing distinct bands. ### Bands: - Clearly visible DNA bands in some lanes suggest successful amplification or presence of specific DNA fragments. - Bands are approximately positioned relative to the markers: - **850 bp Band**: Present in some lanes, indicating a larger DNA fragment. - **550 bp Band**: Also present, signaling smaller DNA fragments. ### Interpretation: - The distinct pattern of bands across different lanes allows for comparison of the DNA samples, evaluating similarities and differences in fragment sizes. This technique is instrumental in applications including genetic analysis, molecular cloning, and forensic studies.
Transcribed Image Text:This image displays a gel electrophoresis result, commonly used for DNA analysis. ### Description of the Gel: - **Lanes:** - The first and last lanes (labeled "Ladder") contain a DNA ladder. This ladder serves as a reference for estimating the size of DNA fragments. Bands are visible at different positions corresponding to known base pair (bp) lengths. - Lanes labeled "LS," "KT," "MV," "DY," and "JY" contain DNA samples being analyzed, each showing distinct bands. ### Bands: - Clearly visible DNA bands in some lanes suggest successful amplification or presence of specific DNA fragments. - Bands are approximately positioned relative to the markers: - **850 bp Band**: Present in some lanes, indicating a larger DNA fragment. - **550 bp Band**: Also present, signaling smaller DNA fragments. ### Interpretation: - The distinct pattern of bands across different lanes allows for comparison of the DNA samples, evaluating similarities and differences in fragment sizes. This technique is instrumental in applications including genetic analysis, molecular cloning, and forensic studies.
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