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1.0 MS2 T4 E. coli 0.5 10-4 10-3 102 10' 1 10 102 10 104 Cot (mole x sec/L) b.) This next figure compares the DNA from two bacteriophages (MS2 and T4) and a bacterium (E. coli). The genome sizes are as follows: MS2 3569bp; T4 168, 903bp; E. coli ~- 600, 000bp. Describe the relationship of the three different curves to each other. ii. Explain this relationship in terms of genome size. i. c.) When half of the DNA in the sample is double-stranded, that point is termed the half- Fraction remaining single-stranded (C/C,)

1.0 MS2 T4 E. coli 0.5 10-4 10-3 102 10' 1 10 102 10 104 Cot (mole x sec/L) b.) This next figure compares the DNA from two bacteriophages (MS2 and T4) and a bacterium (E. coli). The genome sizes are as follows: MS2 3569bp; T4 168, 903bp; E. coli ~- 600, 000bp. Describe the relationship of the three different curves to each other. ii. Explain this relationship in terms of genome size. i. c.) When half of the DNA in the sample is double-stranded, that point is termed the half- Fraction remaining single-stranded (C/C,)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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1.0 MS2 T4 E. coli 0.5 104 103 102 10- 10' 102 103 10* 1 Cot (mole x sec/L) b.) This next figure compares the DNA from two bacteriophages (MS2 and T4) and a bacterium (E. coli). The genome sizes are as follows: MS2 3569bp; T4 168, 903bp; E. coli -~4. 600, 000bp. i. Describe the relationship of the three different curves to each other. ii. Explain this relationship in terms of genome size. c.) When half of the DNA in the sample is double-stranded, that point is termed the half- reaction time (Cot12). Plot a graph below that describes how genome sizes of MS2, T4 and E.coli affects the half-reactioh time. Fraction remaining single-stranded (C/Čo)
Transcribed Image Text:1.0 MS2 T4 E. coli 0.5 104 103 102 10- 10' 102 103 10* 1 Cot (mole x sec/L) b.) This next figure compares the DNA from two bacteriophages (MS2 and T4) and a bacterium (E. coli). The genome sizes are as follows: MS2 3569bp; T4 168, 903bp; E. coli -~4. 600, 000bp. i. Describe the relationship of the three different curves to each other. ii. Explain this relationship in terms of genome size. c.) When half of the DNA in the sample is double-stranded, that point is termed the half- reaction time (Cot12). Plot a graph below that describes how genome sizes of MS2, T4 and E.coli affects the half-reactioh time. Fraction remaining single-stranded (C/Čo)
0.5 104 10 102 10 1 10' 10² 103³ 10* Cot (mole × sec/L) In 1968, Britten and Kohne showed that once double-stranded DNA is dissociated into single- stranded pieces, the rate of reassociation of the complementary strands can be analyzed. For these kinds of experiments, the DNA is first sheared into fragments with an average size of several hundred base pairs. The fragments are dissociated (denatured) via heating, and then the sample temperature is lowered slowly while the reassociation is monitored. During reassociation, pieces of single-stranded molecules collide at random. If the pieces are complementary, a stable double-stranded molecule is formed. If no match is made, the pieces separate and continue encountering other single-stranded pieces. This process continues until all matches are made. a. The figure below shows an ideal time course for reassociation of DNA. Co is the initial concentration of DNA single strands in moles per liter. Time (t) of the reassociation is measured in seconds. C is the concentration of single-stranded DNA remaining after some time, t, has elapsed. For simplicity, you can think about the X-axis as time elapsed. i. At time t = 0, what is the relationship of C vs. Co? ii. You can see the slope of the line changes over the span of the reaction. Describe the slope at the beginning, middle and end of the reaction using the following words: steep and shallow. iii. Explain why the slope is as you describe it at the beginning, middle and end of the reaction. Provide a molecular explanation.
Transcribed Image Text:0.5 104 10 102 10 1 10' 10² 103³ 10* Cot (mole × sec/L) In 1968, Britten and Kohne showed that once double-stranded DNA is dissociated into single- stranded pieces, the rate of reassociation of the complementary strands can be analyzed. For these kinds of experiments, the DNA is first sheared into fragments with an average size of several hundred base pairs. The fragments are dissociated (denatured) via heating, and then the sample temperature is lowered slowly while the reassociation is monitored. During reassociation, pieces of single-stranded molecules collide at random. If the pieces are complementary, a stable double-stranded molecule is formed. If no match is made, the pieces separate and continue encountering other single-stranded pieces. This process continues until all matches are made. a. The figure below shows an ideal time course for reassociation of DNA. Co is the initial concentration of DNA single strands in moles per liter. Time (t) of the reassociation is measured in seconds. C is the concentration of single-stranded DNA remaining after some time, t, has elapsed. For simplicity, you can think about the X-axis as time elapsed. i. At time t = 0, what is the relationship of C vs. Co? ii. You can see the slope of the line changes over the span of the reaction. Describe the slope at the beginning, middle and end of the reaction using the following words: steep and shallow. iii. Explain why the slope is as you describe it at the beginning, middle and end of the reaction. Provide a molecular explanation.
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