pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI: 11 kb, 6 kb, 3 kb EcoRI + BamHI: 8 kb, 6 kb, 3 kb Which would be the most appropriate ladder for the gel that you would pour?
pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI: 11 kb, 6 kb, 3 kb EcoRI + BamHI: 8 kb, 6 kb, 3 kb Which would be the most appropriate ladder for the gel that you would pour?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below:
EcoRI: 20 kb
BamHI: 11 kb, 6 kb, 3 kb
EcoRI + BamHI: 8 kb, 6 kb, 3 kb
Which would be the most appropriate ladder for the gel that you would pour?

Transcribed Image Text:A B C D
DNA Mass
(ng/5μl)
25
67
30
27
30
Base Pairs
||||||||||||||
1500
1200
1000
800
700
600
500
450
400
350
300
250
-200
-150
100
-50
2% TAE agarose gel
900
1% agarose
bp
4,000
3,000
2,000
1,650
1,000
850
650
500
400
300
200
100
XCFF-
Tartrazine-|
bp
-2072
-1500
-900
-600
-300
-100
DNA Mass
(ng/5μl)
16
28
28
28
28
18
92
34
34
20
92
23
30
45
Base Pairs
| | || |
25K
10K
8K
6K
5K
- 4K
- 3K
-2.5K
<<-2K
-1.5K
- 1K
-750
- 500
- 250
0.7% TAE agarose gel
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