Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI: 11 kb, 6 kb, 3 kb EcoRI + BamHI: 8 kb, 6 kb, 3 kb Which would be the most appropriate ladder for the gel that you would pour?

pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI: 11 kb, 6 kb, 3 kb EcoRI + BamHI: 8 kb, 6 kb, 3 kb Which would be the most appropriate ladder for the gel that you would pour?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below:

EcoRI: 20 kb

BamHI: 11 kb, 6 kb, 3 kb

EcoRI + BamHI: 8 kb, 6 kb, 3 kb

Which would be the most appropriate ladder for the gel that you would pour?

A B C D DNA Mass (ng/5μl) 25 67 30 27 30 Base Pairs |||||||||||||| 1500 1200 1000 800 700 600 500 450 400 350 300 250 -200 -150 100 -50 2% TAE agarose gel 900 1% agarose bp 4,000 3,000 2,000 1,650 1,000 850 650 500 400 300 200 100 XCFF- Tartrazine-| bp -2072 -1500 -900 -600 -300 -100 DNA Mass (ng/5μl) 16 28 28 28 28 18 92 34 34 20 92 23 30 45 Base Pairs | | || | 25K 10K 8K 6K 5K - 4K - 3K -2.5K <<-2K -1.5K - 1K -750 - 500 - 250 0.7% TAE agarose gel
Transcribed Image Text:A B C D DNA Mass (ng/5μl) 25 67 30 27 30 Base Pairs |||||||||||||| 1500 1200 1000 800 700 600 500 450 400 350 300 250 -200 -150 100 -50 2% TAE agarose gel 900 1% agarose bp 4,000 3,000 2,000 1,650 1,000 850 650 500 400 300 200 100 XCFF- Tartrazine-| bp -2072 -1500 -900 -600 -300 -100 DNA Mass (ng/5μl) 16 28 28 28 28 18 92 34 34 20 92 23 30 45 Base Pairs | | || | 25K 10K 8K 6K 5K - 4K - 3K -2.5K <<-2K -1.5K - 1K -750 - 500 - 250 0.7% TAE agarose gel
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS