Mild detergents, like Triton X-100, would be the best for isolation of peripheral proteins from the plasma membrane (without denaturing these proteins) because: they are non-polar with mostly negatively-charged regions they are non-polar with mostly positively-charged regions they are non-polar with mostly uncharged regions they are polar with mostly uncharged regions they are polar with mostly charged regions

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Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.