Mild detergents, like Triton X-100, would be the best for isolation of peripheral proteins from the plasma membrane (without denaturing these proteins) because: they are non-polar with mostly negatively-charged regions they are non-polar with mostly positively-charged regions they are non-polar with mostly uncharged regions they are polar with mostly uncharged regions they are polar with mostly charged regions

Mild detergents, like Triton X-100, would be the best for isolation of peripheral proteins from the plasma membrane (without denaturing these proteins) because: they are non-polar with mostly negatively-charged regions they are non-polar with mostly positively-charged regions they are non-polar with mostly uncharged regions they are polar with mostly uncharged regions they are polar with mostly charged regions

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

Problem 2RQ

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Figure 13.6 Which of the following statements is true? a. Gram-positive bacteria have a single cell wall formed from peptidoglycan. b. Gram-positive bacteria have an outer membrane. c. The cell wall of Gram-negative bacteria is thick, and the cell wall of Gram-positive bacteria is thin. d. Gram-negative bacteria have a cell wall made of peptidoglycan, while Gram-positive bacteria have a cell wall made of phospholipids.
Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain surgical procedures, such as those for malignancy, the surgeon may request that sterile water be used for irrigation instead of saline following excision of the cancerous mass. Why is normal saline usually used in healthy tissues?
Antibiotics and Protein Synthesis Antibiotics are molecules produced by microorganisms as defense mechanisms. The most effective antibiotics work by interfering with essential biochemical or reproductive processes. Many antibiotics block or disrupt one or more stages in protein synthesis. Some of these are mentioned here. Tetracyclines are a family of chemically related compounds used to treat several types of bacterial infections. Tetracyclines interfere with the initiation of translation. The tetracycline molecule attaches to the small ribosomal subunit and prevents binding of the tRNA anticodon during initiation. Both eukaryotic and prokaryotic ribosomes are sensitive to the action of tetracycline, but this antibiotic cannot pass through the plasma membrane of eukaryotic cells. Because tetracycline can enter bacterial cells to inhibit protein synthesis, it will stop bacterial growth, helping the immune system fight the infection. Streptomycin is used in hospitals to treat serious bacterial infections. It binds to the small ribosomal subunit but does not prevent initiation or elongation; however, it does affect the efficiency of protein synthesis. Binding of streptomycin changes the way mRNA codons interact with the tRNA. As a result, incorrect amino acids are incorporated into the growing polypeptide chain, producing nonfunctional proteins. In addition, streptomycin causes the ribosome to randomly fall off the mRNA, preventing the synthesis of complete proteins. Puromycin is not used clinically but has played an important role in studying the mechanism of protein synthesis in the research laboratory. The puromycin molecule is the same size and shape as a tRNA/amino acid complex. When puromycin enters the ribosome, it can be incorporated into a growing polypeptide chain, stopping further synthesis because no peptide bond can be formed between puromycin and an amino acid, causing the shortened polypeptide to fall off the ribosome. Chloramphenicol was one of the first broadspectrum antibiotics introduced. Eukaryotic cells are resistant to its actions, and it was widely used to treat bacterial infections. However, its use is limited to external applications and serious infections. Chloramphenicol destroys cells in the bone marrow, the source of all blood cells. In bacteria, this antibiotic binds to the large ribosomal subunit and inhibits the formation of peptide bonds. Another antibiotic, erythromycin, also binds to the large ribosomal subunit and inhibits the movement of ribosomes along the mRNA. Almost every step of protein synthesis can be inhibited by one antibiotic or another. Work on designing new synthetic antibiotics to fight infections is based on our knowledge of how the nucleotide sequence of mRNA is converted into the amino acid sequence of a protein. Questions Why is targeting protein synthesis an effective strategy for preventing infection?
Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain surgical procedures, such as those for malignancy, the surgeon may request that sterile water be used for irrigation instead of saline following excision of the cancerous mass. What reaction will occur between the eukaryotic cells of the body and the water?
Which of the following sequences correctly lists in order the steps involved in the incorporation of a proteinaceous molecule within a cell? protein synthesis of the protein on the ribosome; modification in the Golgi apparatus; packaging in the endoplasmic reticulum; tagging in the vesicle synthesis of the protein on the lysosome; tagging in the Golgi; packaging in the vesicle; distribution in the endoplasmic reticulum synthesis of the protein on the ribosome; modification in the endoplasmic reticulum; tagging in the Golgi; distribution via the vesicle synthesis of the protein on the lysosome; packaging in the vesicle; distribution via the Golgi; tagging in the endoplasmic reticulum
Figure 22.16 Which of the following statements is true? Gram-positive bacteria have a single cell wall anchored to the cell membrane by lipoteichoic acid. Pori ns allow entry of substances into both Gram-positive and Gram-negative bacteria. The cell wall of Gram-negative bacteria is thick, and the cell wall of Gram-positive bacteria is thin. Gram-negative bacteria have a cell wall made of peptidoglycan, whereas Gram positive bacteria have a cell wall made of lipoteichoic acid.
Which of these statements is true? An antibiotic is any substance produced by a organism that is antagonistic to the growth of prokaryotes An antibiotic is any substance produced by a prokaryote that is antagonistic to the growth of other viruses An antibiotic is any substance produced by a prokaryote that is antagonistic to the growth of eukaryotic cells An antibiotic is any substance produced by a prokaryote that prevents growth of the same prokaryote.
Prokaryotes stain as Gram-positive or Gram-negative because of differences in the_______. a. cell wall b. cytoplasm c. nucleus d. chromosome
Why is it advantageous for the cell membrane to be fluid in nature?
There have been recurring cases of mad-cow disease in the United Kingdom since the mid-1990s. Mad-cow disease is caused by a prion, an infectious particle that consists only of protein. In 1986, the media began reporting that cows all over England were dying from a mysterious disease. Initially, there was little interest in determining whether humans could be affected. For 10 years, the British government maintained that this unusual disease could not be transmitted to humans. However, in March 1996, the government did an about-face and announced that bovine spongiform encephalopathy (BSE), commonly known as mad-cow disease, can be transmitted to humans, where it is known as variant Creutzfeldt-Jakob disease (VCJD). As in cows, this disease eats away at the nervous system, destroying the brain and essentially turning it into a spongelike structure filled with holes. Victims experience dementia; confusion; loss of speech, sight, and hearing; convulsions; coma; and finally death. Prion diseases are always fatal, and there is no treatment. Precautionary measures taken in Britain to prevent this disease in humans may have begun too late. Many of the victims contracted it over a decade earlier, when the BSE epidemic began, and the incubation period is long (VCJD has an incubation period of 10 to 40 years). A recent study concluded that 1 in 2,000 people in Great Britain carry the abnormally folded protein that causes VCJD. In spite of these numbers, the death rate from VCJD remains low. It is not clear whether this means that the incubation period for the disease is much longer than previously thought, or whether they may never develop the disease. How can a prion replicate itself without genetic material?
In what situation(s) would a transmission electron microscope be ideal, and why?