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Enzymes BamHI EcoRI HindIII BamHI + EcoRI BamHI + HindIII EcoRI + HindIII BamHI + EcoRI + HindIII Fragment Sizes (in kb) 7.3, 3.2 10.5 5.1, 3.4, 2.0 6.7, 3.2, 0.6 4.6, 2.7, 2.0, 0.7, 0.5 4.0, 3.4, 2.0, 1.1 4.0, 2.7, 2.0, 0.7, 0.6, 0.5 Draw a restriction map for the plasmid that fits your data.

Enzymes BamHI EcoRI HindIII BamHI + EcoRI BamHI + HindIII EcoRI + HindIII BamHI + EcoRI + HindIII Fragment Sizes (in kb) 7.3, 3.2 10.5 5.1, 3.4, 2.0 6.7, 3.2, 0.6 4.6, 2.7, 2.0, 0.7, 0.5 4.0, 3.4, 2.0, 1.1 4.0, 2.7, 2.0, 0.7, 0.6, 0.5 Draw a restriction map for the plasmid that fits your data.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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**Restriction Mapping of Plasmid DNA** *Overview:* You are studying a circular plasmid DNA molecule of size 10.5 kilobase pairs (kb). The plasmid is digested with restriction endonucleases BamHI, EcoRI, and HindIII, both individually and in all possible combinations. The resulting linear restriction fragments have the following sizes: *Table of Enzymes and Fragment Sizes:* | Enzymes | Fragment Sizes (in kb) | |-----------------------------|---------------------------| | **BamHI** | 7.3, 3.2 | | **EcoRI** | 10.5 | | **HindIII** | 5.1, 3.4, 2.0 | | **BamHI + EcoRI** | 6.7, 3.2, 0.6 | | **BamHI + HindIII** | 4.6, 2.7, 2.0, 0.7, 0.5 | | **EcoRI + HindIII** | 4.0, 3.4, 2.0, 1.1 | | **BamHI + EcoRI + HindIII** | 4.0, 2.7, 2.0, 0.7, 0.6, 0.5 | *Task:* Draw a restriction map for the plasmid that fits your data. *Explanation:* - **BamHI** digestion results in two fragments of 7.3 kb and 3.2 kb, indicating one BamHI site on the plasmid. - **EcoRI** produces a single fragment of 10.5 kb, suggesting a single EcoRI site. - **HindIII** cuts the plasmid into three fragments of 5.1 kb, 3.4 kb, and 2.0 kb, indicating multiple HindIII sites. - Combinations of enzymes further dissect these fragments, as shown in the table, which aids in mapping the relative positions of restriction sites on the plasmid. Understanding the arrangement of these sites allows for the comparison of these fragments and their combinations to map out the specific locations of each enzyme site on the plasmid DNA.
Transcribed Image Text:**Restriction Mapping of Plasmid DNA** *Overview:* You are studying a circular plasmid DNA molecule of size 10.5 kilobase pairs (kb). The plasmid is digested with restriction endonucleases BamHI, EcoRI, and HindIII, both individually and in all possible combinations. The resulting linear restriction fragments have the following sizes: *Table of Enzymes and Fragment Sizes:* | Enzymes | Fragment Sizes (in kb) | |-----------------------------|---------------------------| | **BamHI** | 7.3, 3.2 | | **EcoRI** | 10.5 | | **HindIII** | 5.1, 3.4, 2.0 | | **BamHI + EcoRI** | 6.7, 3.2, 0.6 | | **BamHI + HindIII** | 4.6, 2.7, 2.0, 0.7, 0.5 | | **EcoRI + HindIII** | 4.0, 3.4, 2.0, 1.1 | | **BamHI + EcoRI + HindIII** | 4.0, 2.7, 2.0, 0.7, 0.6, 0.5 | *Task:* Draw a restriction map for the plasmid that fits your data. *Explanation:* - **BamHI** digestion results in two fragments of 7.3 kb and 3.2 kb, indicating one BamHI site on the plasmid. - **EcoRI** produces a single fragment of 10.5 kb, suggesting a single EcoRI site. - **HindIII** cuts the plasmid into three fragments of 5.1 kb, 3.4 kb, and 2.0 kb, indicating multiple HindIII sites. - Combinations of enzymes further dissect these fragments, as shown in the table, which aids in mapping the relative positions of restriction sites on the plasmid. Understanding the arrangement of these sites allows for the comparison of these fragments and their combinations to map out the specific locations of each enzyme site on the plasmid DNA.
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