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Which of the following statements describe disadvantages of the classical Sanger sequencing approach when compared to the modern Sanger sequencing method? Radioactivity must be used, representing an added hazard Fluorescence is used, representing an added hazard O Four reaction are used, requiring more reagents and escalating cost O Gel electrophoresis is used to separate the various products by size but slowing overall completion of sequencing OA single reaction is used, complicating the analysis O Capillary electrophoresis is used to separate the various products by size but slowing overall completion of sequencing

Which of the following statements describe disadvantages of the classical Sanger sequencing approach when compared to the modern Sanger sequencing method? Radioactivity must be used, representing an added hazard Fluorescence is used, representing an added hazard O Four reaction are used, requiring more reagents and escalating cost O Gel electrophoresis is used to separate the various products by size but slowing overall completion of sequencing OA single reaction is used, complicating the analysis O Capillary electrophoresis is used to separate the various products by size but slowing overall completion of sequencing

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 3 Listen Which of the following statements describe disadvantages of the classical Sanger sequencing approach when compared to the modern Sanger sequencing method? |Radioactivity must be used, representing an added hazard Fluorescence is used, representing an added hazard Four reaction are used, requiring more reagents and escalating cost OGel electrophoresis is used to separate the various products by size but slowing overall completion of sequencing OA single reaction is used, complicating the analysis O Capillary electrophoresis is used to separate the various products by size but slowing overall completion of sequencing Question 4 4 Listen Electrophoresis of polynucleotides can be done using either agarose or polyacrylamide gels. What is the primary consideration when choosing which polymer gel to use? O Overall charge on polynucleotide sample, greater overall negative charge requires agarose gel with smaller pores to slow the migration of the highly charged sample O Overall charge on polynucleotide sample, greater overall negative charge requires polyacrylamide gel with smaller pores to slow the migration of the highly charged sample OOverall length of polynucleotide sample, greater overall length requires agarose gel with larger pores to allow the migration of large polynucleotide chains Overall length of polynucleotide sample, greater overall length requires polyacrylamide gel with larger pores to allow the migration of large polynucleotide chains
Transcribed Image Text:Question 3 Listen Which of the following statements describe disadvantages of the classical Sanger sequencing approach when compared to the modern Sanger sequencing method? |Radioactivity must be used, representing an added hazard Fluorescence is used, representing an added hazard Four reaction are used, requiring more reagents and escalating cost OGel electrophoresis is used to separate the various products by size but slowing overall completion of sequencing OA single reaction is used, complicating the analysis O Capillary electrophoresis is used to separate the various products by size but slowing overall completion of sequencing Question 4 4 Listen Electrophoresis of polynucleotides can be done using either agarose or polyacrylamide gels. What is the primary consideration when choosing which polymer gel to use? O Overall charge on polynucleotide sample, greater overall negative charge requires agarose gel with smaller pores to slow the migration of the highly charged sample O Overall charge on polynucleotide sample, greater overall negative charge requires polyacrylamide gel with smaller pores to slow the migration of the highly charged sample OOverall length of polynucleotide sample, greater overall length requires agarose gel with larger pores to allow the migration of large polynucleotide chains Overall length of polynucleotide sample, greater overall length requires polyacrylamide gel with larger pores to allow the migration of large polynucleotide chains
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