Draw your own PCR diagram to show the role of each component and relevance of each temperature shift during the PCR reaction.What’s happening at each of those temperatures and why is it important it changes? Important points to follow:• Samples S1 and 52 are at a suitable dilution already• PCR reactions are 'run' in the 0.2ml tubes - these should not be squeezed asthey crack.• Each PCR reactions each need to contain:ComponentGoTaq Hot Start Master Mix, 2XPrimer set (E or H; 15ng/μl)DNA to test (P1, S1 or $2; 20ng/μl)Nuclease-free waterYou will set up four reactions.95°C 5 minutes95°C 30 sec50°C 1 minLabel the tubes with details of the DNA or tube number and your workstationnumber so you know which is which next week. Once you've added the necessaryreagents, place your tubes in the correct rack at the front of the lab.We will then cycle these through the following temperatures:1xFinal Volume25.0μl40x5.0μl1.0μlup to 50μl72°C 1 min72°C 10 minThey will then be stored in the fridge (4°C) until you need them next week.

The polymerase chain reaction or PCR is a method widely used to rapidly make multiple copies of a…

Draw your own PCR diagram to show the role of each component and relevance of each temperature shift during the PCR reaction. What’s happening at each of those temperatures and why is it important it changes?

Draw your own PCR diagram to show the role of each component and relevance of each temperature shift during the PCR reaction. What’s happening at each of those temperatures and why is it important it changes?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter14: Biotechnology And Society

Section14.6: Dna Profiles As Tools For Identification

Problem 2EG

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DNA Profiles as Tools for Identification STRs are: a. used for DNA profiles b. repeated sequences present in the human genome c. highly variable in copy number d. all of these e. none of these
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA ligase _____ cDNA synthesis c. reverse transcriptase _____ DNA sequencing d. restriction enzyme _____ pasting DNA e. DNA polymerase (not Taq)
PCR can be used ______. a. as a cloning vector b. in DNA profiling c. to modify a human genome
During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicase
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Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymes
Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.
Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’
What information and materials are needed to amplify region of DNA using PCR?
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Genetics in Practice case studies are critical-thinking exercises that allow you to apply your new knowledge of human genetics to real-life problems. Case study Michelle was a 42-year-old woman who had declined counselling and amniocentesis at 16 weeks of pregnancy but was referred for genetic counseling after an abnormal ultrasound at 20 weeks of gestation. After the ultrasound, a number of findings suggested a possible chromosome abnormality in the fetus. The ultrasound showed swelling under the skin at the back of the fetuss neck; shortness of the femur, humerus, and ear length; and underdevelopment of the middle section of the fifth finger. Michelles physician performed an amniocentesis and referred her to the genetics program. Michelle and her husband did not want genetic counseling before receiving the results of the cytogenetic analysis. This was Michelles third pregnancy; she and her husband, Mike, had a 6-year-old daughter and a 3-year-old son. At their next session, the counselor informed the couple that the results revealed trisomy 21, explored their understanding of Down syndrome, and elicited their experiences with people with disabilities. She also reviewed the clinical concerns revealed by the ultrasound and associated anomalies (mild to severe intellectual disability, cardiac defects, and kidney problems). The options available to the couple were outlined. They were provided with a booklet written for parents making choices after the prenatal diagnosis of Down syndrome. After a week of careful deliberation with their family, friends, and clergy, they elected to terminate the pregnancy. Should physicians discourage a 42-year-old woman from having children because of an increased chance of a chromosomal abnormality?