Which of the following statements is true of a Sanger sequencing reaction but not colony PCR? Select all that apply. Must contain only a single primer ddNTPs are added Uses a heat stable DNA polymerase Requires a pair of primers that bind to opposite strands Requires temperature cycling
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
Which of the following statements is true of a Sanger sequencing reaction but not colony PCR? Select all that apply.
Must contain only a single primer
ddNTPs are added
Uses a heat stable DNA polymerase
Requires a pair of primers that bind to opposite strands
Requires temperature cycling
Sanger sequencing, also known as chain-termination sequencing, is a method for determining the sequence of nucleotide bases in a DNA molecule. The method uses a DNA polymerase to synthesize a complementary strand of DNA using a single-stranded template DNA, which is annealed with a specific primer. In each cycle of the reaction, a dideoxynucleotide (ddNTP) is incorporated into the growing DNA strand, which terminates chain elongation. The resulting DNA fragments are separated by size using gel electrophoresis, and the sequence is read by determining the order of the terminating nucleotides. The method is named after its inventor, Frederick Sanger, and it was the first DNA sequencing technique to be widely used.
A molecular biology technique called colony PCR is used to amplify certain DNA fragments from bacterial colonies. It is frequently used to examine bacterial colonies following plasmid transformation or to confirm the presence of a specific DNA insert in a cloned plasmid.
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
