Q: Which bacterial enzyme removes the primers?a. Primaseb. DNA polymerase Ic. DNA polymerase IIId.…
A: DNA is the double-stranded molecule that is the genetic material in most animals except for some…
Q: DNA can be separated based on fragment size using ________.
A: DNA is Deoxy-Ribo-Nucleic acid and is made by two polynucleotide chains coiled together Structure…
Q: Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and…
A: Restriction digestion is the process of cutting the DNA sequence at a particular nucleotide sequence…
Q: Genetically engineered human insulin, human growth hormone, and human clotting factor VIII are made…
A: Answer is c.) transgenic bacteria.
Q: Which of the following enzyme is required for end to end joining of DNA?a) DNA ligaseb) Restriction…
A: DNA (deoxyribonucleic acid) is a biological macromolecule. It consists of repeating units of…
Q: True or False. During gel electrophoresis, small DNA fragments are found towards the top of the gel…
A: Gel electrophoresis is a technique that uses size and charge to separate and analyze macromolecules…
Q: Instructions In this image, the DNA fragments traveled from top to bottom. Use the drawpad to…
A: DNA is a double stranded helical molecule that is made up of repeating nucleotides. The nucleotides…
Q: A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments…
A: Electrophoresis is a technique used to separate charged particles like protein, RNA, and DNA. It is…
Q: Sanger Illumina PacBio Amount of DNA needed for sequencing Read length Amount of data sequenced…
A: DNA sequencing DNA sequencing involves various techniques by which the order of nucleic acid…
Q: Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells…
A: Polymerase Chain Reaction is a method used to make millions of copies of a specific DNA sample.…
Q: Electrophoresis separates fragments of DNA according to_____ . a. sequence b. length c. species
A: Deoxyribonucleic acid (DNA) is a genetic material and it carries genetic information from one cell…
Q: DNA Extraction Using household materials Wash the fruit. Place the fruit (e.g. banana) in a zip…
A: The molecular biology applications involve protein and nucleic acid isolations. The process involves…
Q: PCR technique is based on DNA transcription. True False
A: Introduction : PCR (polymerase chain reaction), the reaction mixture includes the DNA extract…
Q: •DNA structure •DNA isolation •DNA isolation methods DNA purification urifi
A: DNA, or deoxyribonucleic acid, is the genetic material in people and in other remaining life forms.…
Q: Drag and drop the appropriate text in the gaps below. Firstly, the plasmid DNA in the E. coli is…
A: Joshua Lederberg created the term "plasmid" in 1952. Plasmids are genetic elements which are…
Q: What are restriction enzymes and what do they do? Complete the chart Driginal DNA sequence 5' TGA…
A: Restriction enzymes bind and cleave double-stranded DNA. There are three categories of restriction…
Q: Select all that are true about DNA gel electrophoresis O This technique can be used to separate DNA…
A: The gel electrophoresis is routinely used technique in molecular biology laboratories that helps in…
Q: DNA saquance of interest DNA of interest Open vector Vector containing DNA of interest -Vector DNA…
A: When a genetically changed vector is injected and integrated into the genome of an organism (host),…
Q: Loading dye stains the DNA molecules and thus help to visualize it under UV light apparatus. O True…
A: DNA Loading dye is used to monitor the migration of DNA into the gel during electrophoresis.…
Q: Proteomics analyses samples using electrophoresis and mass spectrometry. True False
A: Proteomic analysis which is also known as proteomics is referred as the branch in which the…
Q: DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette…
A: DNA is the nucleic acid and it absorbs the UV light with maximum absorbance at 260 nm wavelength due…
Q: The production in a pcr reaction is: nucleotids double strand circular dna double stranf linear…
A: PCR stands for polymerase chain reaction.
Q: Once the RNA primers are replaced the fragments in the lagging strands are sealed by DNA pol I.…
A: The flow of genetic information from DNA to RNA, RNA to protein synthesis is called central dogma.…
Q: A DNA sample was sent off for Sanger sequencing. The results are shown below. What is the sequence…
A: The Sanger sequencing is also known as the chain termination method, it is a method used to…
Q: WHOLE CHROMOSOME ARE NOT ANALYZED IN DNA FINGERPRINTING METHOD.
A: Deoxy ribonucleic acid or DNA fingerprinting is a way to identify a certain individual rather than…
Q: ligated into plasmid vectors and transformed into bacterial cells/colonies on agar plates. This…
A: Answer is (D) Gene Library A gene library is a collection of total genomic DNA from an organism in…
Q: The solid matrix that is usually used to attach the target DNA in southern blotting technique is…
A: Blotting is utilized in atomic science for the distinguishing proof of proteins and nucleic acids…
Q: A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments…
A: Gel electrophoresis is a technique used to separate different pieces of DNA on the basis of their…
Q: Replicate the DNA strand AAGGCTAACGGCATTTAACCC. Transcribe the DNA strand AAGGCTAACGGCATTTAACCC.…
A: In most organisms, genetic material is stored in the form of DNA. In humans, each cell's nucleus has…
Q: Illustrate the steps and other methods involved in recombinant DNA
A: Recombinant DNA technology is the latest and advanced technique in the molecular biology and…
Q: Order the following from 1-12 events chronologically based on the next generation sequencing…
A: Genome sequencing is a biotechnological technique which is prominently used in sequencing the genome…
Q: Draw an Illustration of the steps and other methods involved in recombinant DNA ( Please make it…
A: Recombinant DNA is a type of DNA that is made up of sequences from various sources. The steps in the…
Q: Replicate, transcribe and translate the DNA strand below. Be sure to label your 5’ and 3’ ends and…
A: DNA has two strand, one strand is actively used as a template in the transcription process, this is…
Q: Gel electrophoresis separates the DNA fragments according to ____. a. their type b. their…
A: Gel electrophoresis separates the DNA fragments based on their molecular size. The most high…
Q: Select the characteristics/descriptions of DNA polymerase. Select ALL that apply requires a primer…
A: DNA is the genetic material of almost all the organisms, except few viruses and is present in the…
Q: DNA can be electrophoresed. all cells have RNA. DNA polymerase will replicate any bacterial DNA. all…
A: PCR is short for Polymerase Chain Reaction.
Q: In gel electrophoresis, DNA fragments migrate toward the _______electrode; the _______the fragment,…
A: Gel electrophoresis is a technique used to separate DNA molecules on agarose gel on the basis of…
Q: Sample Gel Electrophoresis: A brother and sister's DNA are cut with the same restriction enzyme and…
A: Gel electrophoresis is a technique used to separate DNA fragments according to their size.
Q: step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does…
A: Introduction: Pure DNA is needed for DNA analysis. In cells, DNA can be found. However, cells also…
Q: (kbp) (bp) 4. To the right is an image of an agarose gel. Lanes 1 and 4 are DNA markers (DNA sizes…
A: Agarose gel electrophoresis is the process by which DNA fragments are run on the basis of their…
Q: The proofreading of DNA is essential for faithful replication. True False
A: The process of replication is carried out with the help of a number of enzymes such as helicase, DNA…
Q: During the second step of PCR, the temperature is lowered in order to: denature the taq polymerase…
A: The term 'PCR' stands for Polymerase chain reaction wherein a single DNA fragment can be used as a…
Q: Create a 75-nitrogenous base long DNA and replicate it completely. Make sure initiation, elongation…
A: Replication of DNA : DNA replication is the process where a new strand exactly similar to the…
Q: CCAGAGGTC Replicate this DNA (answer in CAPS)
A:
Q: DNA EXTRACTION Materials: knife 1…
A: DNA extraction: It is the method used for the plants and animals both. It is the most recent and…
Q: DATAAND RESULTS: A. Description and source of isolates Nudeic Acid Source Description BANANA DNA…
A: All living organisms use the same basic molecules.e., DNA (deoxyribonucleic acid) to pass…
Q: Which of the following cuts DNA like molecular scissors? Clustered regularly interpaced short…
A: Clustered regularly interspaced short palindromic repeats that are known as CRISPR are family of DNA…
Q: Whole chromosome are not analyzed in DNA fingerprinting method. Explain.
A: Introduction: DNA is a genetic materiel of living organism except in some viruses. DNA…
Q: In which reagent is extracted DNA suspended to put it in solution? O Sodium dodecyl sulfate (SDS) O…
A: Introduction DNA extraction is a method to isolate DNA in a biological sample, which is done by…
DNA Cheek Cells Lab
Identify the component of the solution which is the extracted DNA
Step by step
Solved in 2 steps
- Select all that are true about DNA gel electrophoresis O This technique can be used to separate DNA based on the size of the fragment O Since DNA is positively charged it will travel to the negative electrode O After a gel is run, the larger fragments end up being further away from the well The rate at which DNA fragments travel is logarithmically and is inversely proportional to its sizeWhat is the purpose of using the Chelex beads while extracting the DNA at high temperature from your cheek cells? Group of answer choices The Chelex binds cations like Mg2+ that are co-factors for nuclease enzymes which would otherwise degrade the DNA. The Chelex denatures the DNA and makes the DNA single stranded. The Chelex will bind the DNA tightly after the cells are ruptured, helping extract it from the cells. The Chelex provides Mg2+ co-factors needed by DNA polymerase enzymes to make more copies of DNADuring agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricity
- Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…DNA EXTRACTION Why do scientists isolate DNA? From what part of the subject’s body were cells collected? Give the specific purpose of each experimental step: Lysis solution & heat: Salt & centrifugation: After centrifuging, where in the tube is the DNA? What is the purpose of adding alcohol?Which well(A through E) of this agarose gel contains the smallest DNA size? Please look at the pic.
- DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?Gel Electrophoresis Background and Protocol: Gel electrophoresis is a laboratory technique that separates molecules by size using an electric current. The test has a positive and negative side. Do you believe DNA should be loaded on the positive (red) side or the negative (black) side? Please explain why using scientific reasoning. Will larger DNA bands be closer or farther away from the well where you administered samples? Why?Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.
- Show the separation pattern of the following DNA molecules on 1 % agarose gel. Justify your results. 5 kbp 5 kbp 5 kbp 5 kb +oner micro mole of 48 nulceotide dna was synthesised via solid phase .this sample contains salts and dna strands in different lenghts(32-48)nt.what can be possible procedure and instruments to purify and conform only 48 nt dna from the mixture.In order to determine the purity of a DNA sample. spectrophotometry can be carried out at calculated, and a number between to measure DNA, and to measure protein. The ratio is then indicates higher purity. 280 nm; 260 nm; A280/A260; 0.5-1 260 nm; 280 nm; A260/A280; 0.5-1 260 nm; 280 nm; A280/A260; 1.5-2 260 nm; 280 nm; A260/A280; 1.5-2