You are studying the DNA binding protein CLAMP and you want to determine its bindingaffinity for different DNA constructs, what technique should you use?ELISAPCRChromatographyWestern BlottingFluorescence polarization

Introduction DNA acts as genetic material in our body. It is a double stranded molecule. mRNA is…

Answered: You are studying the DNA binding…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Why amplify DNA using PCR before doing electrophoresis? Why not just take your DNA sample and directly run a gel?
The idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)
describe the two main parts of a plasmid DNA extraction and purification procedure including the purpose of each step
Sample Gel Electrophoresis: A brother and sister's DNA are cut with the same restriction enzyme and then the resulting DNA fragments are run on a gel next to each other. DRAW THE BANDS on the gel where they would appear: Carissa's DNA was cut into 4 pieces: The pieces were 20 base pairs long, 10bp long, 8 and 5. Christopher's DNA was cut into 5 pieces: They were 25 bp long, 10, 8 and the last two pieces were both 2 bp long. You can make a scole to helo you> 13. What did you do about the 2 DNA fragments that were both 5 base pairs long? 14. How many DNA fragment sizes do these kids have in common? Carissa M Christopher Smith
The schematic on the right is for which molecular biology method? What information does this method reveal?
Select the methods that are currently used in Covid tests Gel electrophoresis PCR Sanger Sequencing RT-PCR CRISPR-Cas Sanger Sequencing. You selected this answer
What type of experiment would you be most likely to use to demonstrate that protein X was directly phosphorylated by the M-cyclin/CDK pair a flow cytometry experiment a PCR based assay an in vitro assay a fluorescence microscopy experiment

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS

You are studying the DNA binding protein CLAMP and you want to determine its binding affinity for different DNA constructs, what technique should you use? ELISA PCR Chromatography Western Blotting Fluorescence polarization