Which of the following are necessary for Sanger sequencing? Select all correct answers. Group of answer choices ddNTPs an oligonucleotide primer DNA polymerase dNTPs DNA ligase a restriction enzyme

Q: Match the term in column 2 (A-G) with its definition in column 1. DOD Cloning vector Genome editing…

A: In the molecular biology, various techniques empower scientists to unravel the mysteries encoded…

Q: What is the role of the plasmid ? Explain the experimental details of the transformation. How can…

A: Recombinant DNA technology is also known as genetic engineering. It involves the manipulation/…

Q: Identify types of plasmid( nicked, linear, supercolied and circular,stranded on each band

A: Answer: PLASMID DNA : It is the small, extrachromosomal DNA found in most bacteria and archea. It is…

Q: che wild type DNA sequence reads THE CAT ATE THE BIG RAT, what type of mutation would change the…

A: Any heritable permanent change in the DNA (deoxyribonucleic acid) sequence is referred to as a…

Q: Select all features that are part of Okazaki fragments.   They are checked for accuracy by DNA…

A: The ability to synthesize both of the daughter strands necessary for cell division makes Okazaki…

Q: PCR primers Below is a 300 base pair fragment of DNA. The top strand is written in the 5' to 3'…

A: The PCR is known as a polymerase chain reaction in which the DNA template is amplified with the help…

Q: At the end of the procedure, what do you do with the cloudy, stringy mass of DNA in order to observe…

A: Here, B) Quickly centrifuging the DNA is the right answer. Depending on the technique of observation…

Q: A cloned fragment of DNA was sequenced by using the dideoxy method. A part of the autoradiogram of…

A: Sanger sequencing, often called chain-termination sequencing, is a DNA sequencing technology…

Q: After properly troubleshooting, you finally are able to visualize the gel. You obtained the image…

A: DNA extraction is a protocol of isolating genomic DNA from any cell type. Generally we use the…

Q: What is the fastest method to determine if a genetic disorder is due to a mutation at a palindromic…

A: Mutations This refers to the alterations in the sequence of DNA. These are caused from the mistakes…

Q: The right-hand column contains descriptions of 5 types of molecular markers. Label each description…

A: These markers are pivotal tools within the field of genetics and genomics, helping researchers…

Q: Chironomus genomic DNA contains approximately 35.5% A. Estimate the percentages of G, C, and T in…

A: The double (ds) stranded DNA duplex structure follows the chargaffs rule, which suggests that the A…

Q: A fragment of DNA that was cut with a restriction enzyme has a 3’ overhang. This overhang is…

A: Restriction Enzymes: These enzymes identify certain DNA sequences, known as recognition or…

Q: gment shows where a number c between those cut sites in base Bem het Bem H 700 800 1000 1100

A: A restriction enzyme, also known as a restriction endonuclease or restrictase, is an enzyme that…

Q: A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes. EcoRI cuts at…

A: In this question, we are given two restriction enzymes EcoRI and HindIII with their restricting…

Q: following question is related to Restriction Enzymes and RFLP. Using EcoRl, which DNA sequence held…

A: Restriction enzymes, also known as restriction endonucleases, are a key tool in molecular biology.…

Q: Order the following steps according to the Sanger sequencing method The bands in the electrophoresis…

A: DNA sequencing is a technique used to evaluate the exact nucleotide bases , their position as well…

Q: A student wanted to load .75 ug dna on agarose gel and has 4x loading buffer for sample preparation.…

A: Agarose gel electrophoresis is a method used for the separation of DNA fragments which is stained by…

Q: Which of the following will lead to DNA cutting more frequently? Choose all that apply.

A: The restriction enzymes are enzymes that cut the DNA at specific nucleotide sequences called the…

Q: Select all the following statements that are correct regarding agarose gel electrophoresis and…

A: Agarose gel electrophoresis is a process used for separation of DNA fragments. Steps of agarose gel…

Q: Which of the following is not a component of Sanger sequencing? OddNTPs with fluorescent tags…

A: Question 1 explantion: The component not typically used in Sanger sequencing is restriction enzymes.…

Q: The diagram (below) shows the completed gel of a Sanger sequencing reaction. The arrows at the…

A: gel electrophoresis is a process in which the negatively charged DNA molecule is separated according…

Q: The production in a pcr reaction is: nucleotids double strand circular dna double stranf linear…

A: PCR stands for polymerase chain reaction.

Q: Which of the following is/are true regarding a PCR reaction performed to amplify a DNA plasmid?…

A: PCR or polymerase chain reaction is a molecular technique which is used to quickly multiply a given…

Q: Write the process of, building a recombinant plasmid? Or how you can build a recombinant plasmid?

A: A plasmid is a circular, single-stranded DNA molecule that is distinct from a cell's chromosomal…

Q: Name the bacterial enzyme that takes over new strand synthesis in replication after the primer is…

A: The process in which the genetic material of an organism (DNA in this case) is duplicated is called…

Q: The following picture shows the ethidium bromide-stained bands obtained by gel electrophoresis of…

A: Electrophoresis is a molecular technique that employs the electrical field to assort biomolecules,…

Q: Why RT-PCR is important in the sample preparation to perform expression microarray experiment?

A: RT - PCR : Reverse transcription- polymerase chain reaction is one of the types of PCR.  DNA…

Q: The enzyme that removes the RNA primer from the Okazaki fragment is:   DNA pol III   DNA ligase…

A: Introduction: DNA is the hereditary material that defines every cell. It is found within the nucleus…

Q: PCR primers Below is a 300 base pair fragment of DNA. The top strand is written in the 5' to 3'…

A: Introduction A primer is a single-stranded nucleic acid that all living organisms utilise to start…

Q: Electrophoresis data: Measure the distance (in millimeters) that each fragment traveled from the…

A: Introduction Gel electrophoresis is a laboratory technique for separating DNA, RNA, and protein…

Q: Above are the results of gel electrophoresis following digestion with restriction enzymes. What is…

A: Using specialized enzymes known as restriction endonucleases, restriction digestion is a laboratory…

Q: Which enzyme can recognize an altered base in the DNA? Group of answer choices DNA Polymerase AP…

A: There are several DNA repair mechanisms are present that are- direct repair system, excision repair…

Q: DNA TEMPLATE: 3 'GCA TTT GAT AAA TAC CTG AGA TGA CTG ATT GGG GGC AAA 5' 5' CGT AAA CTA TTT ATG GAC…

A:

Q: A DNA fingerprint consists of sections of DNA that have been cut with restriction endonuclease…

A: DNA fingerprinting DNA fingerprinting is a modern technique that help us to solve  crimes. The…

Q: Which of the following is required in running a PCR reaction? Promoter Primase DNTPS DNA polymerase…

A: PCR is polymerase chain reaction , which is a biotechnology tool used in DNA amplification.

Q: You are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this…

A: Gel electrophoresis is the molecular biology technique used to separate the DNA fragments on the…

Q: Draw an Illustration of the steps and other methods involved in recombinant DNA ( Please make it…

A: Recombinant DNA is a type of DNA that is made up of sequences from various sources. The steps in the…

Q: Which two restriction enzymes could be used to cut this DNA sequence?…

A: Restriction enzymes are proteins that recognize specific DNA sequences and cut the DNA at or near…

Q: Write a short discussion based on the result above.

A: The peak at 260nm is significantly important here. The value of 260/280 and 260/230 helps in…

Q: Which of the following components are required for the polymerase chain reaction (PCR)? Select all…

A: The components that are required for PCR are-  dNTPs, DNA polymerase, Oligonucleotide primers

Q: Use the sequence provided and make use of figure 1 to determine what restriction enzyme uses the…

A: The lambda phage is a Escherichia coli bacteriophage. The virion DNA is linear and double-stranded…

Q: Your next task is to study Ebola virus (EboV) sequences of different strains. You have received…

A: Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to…