a) In lane 5, draw what you would expect to see if you prepared a reaction using a nucleotide mix containing only dATP, DTTP, dCTP, and dGTP. b) In lane 6, draw what you would expect to see if you prepared a reaction using a nucleotide mix containing only ddATP, dTTP, dCTP, AGTP.
a) In lane 5, draw what you would expect to see if you prepared a reaction using a nucleotide mix containing only dATP, DTTP, dCTP, and dGTP. b) In lane 6, draw what you would expect to see if you prepared a reaction using a nucleotide mix containing only ddATP, dTTP, dCTP, AGTP.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:1. You decide to perform dideoxy sequencing on a PCR product. You add the appropriate 32P-
labeled primer, DNA polymerase, DNA template (the PCR product), buffer, DNTP mix, and a
small amount of one of the four ddNTPs to four reaction tubes. You run the reactions in the
thermal cycler, load each reaction into a separate lane of a polyacrylamide gel, and separate
the products by gel electrophoresis. In the figure below, the lanes are labeled according to
the ddNTP added.
ddATP ddTTP ddCTP ddGTP
-
Lane 1
2
3
4
a) In lane 5, draw what you would expect to see if you prepared a reaction using a nucleotide
mix containing only DATP, DTTP, dCIP, and dGTP.
b) In lane 6, draw what you would expect to see if you prepared a reaction using a nucleotide
mix containing only ddATP, DTTP, dCIP, dGTP.
||
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