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Why is Sanger sequencing referred to as the chain termination method? Select one: a. Sugars on ddNTP lack 3’OH, which prevents the binding of the next nucleotide b. Sugars on the ddNTP have an OH in the 3', which catalyzes the cleavage of phosphodiester bonds c. Sugars on ddNTP lack 5' OH, which prevents the binding of the next nucleotide d. Bases on ddNTP lack 3’ OH, which prevents the binding of the next nucleotide е. Bases on ddNTP lack 5' OH, which catalyzes the cleavage of the phosphodiester bond

Why is Sanger sequencing referred to as the chain termination method? Select one: a. Sugars on ddNTP lack 3’OH, which prevents the binding of the next nucleotide b. Sugars on the ddNTP have an OH in the 3', which catalyzes the cleavage of phosphodiester bonds c. Sugars on ddNTP lack 5' OH, which prevents the binding of the next nucleotide d. Bases on ddNTP lack 3’ OH, which prevents the binding of the next nucleotide е. Bases on ddNTP lack 5' OH, which catalyzes the cleavage of the phosphodiester bond

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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**Why is Sanger sequencing referred to as the chain termination method?** Select one: - a. Sugars on ddNTP lack 3’ OH, which prevents the binding of the next nucleotide - b. Sugars on the ddNTP have an OH in the 3’, which catalyzes the cleavage of phosphodiester bonds - c. Sugars on ddNTP lack 5’ OH, which prevents the binding of the next nucleotide - d. Bases on ddNTP lack 3’ OH, which prevents the binding of the next nucleotide - e. Bases on ddNTP lack 5’ OH, which catalyzes the cleavage of the phosphodiester bond
Transcribed Image Text:**Why is Sanger sequencing referred to as the chain termination method?** Select one: - a. Sugars on ddNTP lack 3’ OH, which prevents the binding of the next nucleotide - b. Sugars on the ddNTP have an OH in the 3’, which catalyzes the cleavage of phosphodiester bonds - c. Sugars on ddNTP lack 5’ OH, which prevents the binding of the next nucleotide - d. Bases on ddNTP lack 3’ OH, which prevents the binding of the next nucleotide - e. Bases on ddNTP lack 5’ OH, which catalyzes the cleavage of the phosphodiester bond
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