Scenario 1: DNA concentration? UV-Vis spectroscopy [Choose ] Homology Modeling Scenario 2: Does it form a tetramer? 2-D NMR Fluorescence Circular Dichroism Cryo-EM DNA sequencing Scenario 2: Is the structure different SAXS X-crystallography UV-Vis spectroscopy Mass Spectrometry FRET Scenario 3: Biomolecule unfolding Scenario 4: Is it a viable protein? Homology Modeling >
For each scenario below, pick which technique you might use to answer the questiion
1. You have received a new shipment of DNA from a collaborator and need to determine the concentration of the DNA before using it.
2. You have identified a mutant in the oxygen carrying protein, Hemoglobin, which you believe alters the protein from forming a tetramer. You want to determine whether it forms a tetramer and if the overall structure has changed. (Probably need two different techniques here)
3. You want to determine whether the protein I-contain-tryptophan2 is unfolding in solution with the addition of a drug-like molecule.
4. You have identified the sequence of a new gene, but are unsure of what the resulting product might look like and if it would be a viable protein-coding gene
![Scenario 1: DNA concentration?
UV-Vis spectroscopy
[Choose ]
Homology Modeling
2-D NMR
Scenario 2: Does it form a tetramer?
Fluorescence
Circular Dichroism
Scenario 2: Is the structure different
Cryo-EM
DNA sequencing
SAXS
X-crystallography
UV-Vis spectroscopy
Mass Spectrometry
Scenario 3: Biomolecule unfolding
FRET
Scenario 4: Is it a viable protein?
Homology Modeling](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9fa0e0b4-1d15-4ef2-8096-9bb34c375057%2F4e82acd6-3b97-4ef3-b134-d0eab93c1932%2Fzvz983o_processed.png&w=3840&q=75)
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