Agarose gels with different average pore sizes areneeded to separate DNA molecules of different sizeclasses. For example, optimal separation of 1100 bpand 1200 bp fragments would require a gel with alarger average pore size than optimal separation of8500 bp and 8600 bp fragments. How do you thinkthat scientists prepare gels of different average poresizes? (Hint: Agarose gels are made in a mannersimilar to gelatin desserts such as JELL-O.)

Q: B. How many non-covalent hydrogen bonds stabilize this structure? 8 C. How many covalent phosphate…

A: The trinucleotide sequence is:5' - ACG - 3'The complementary trinucleotide sequence will be:3' - TGC…

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A: DNA or deoxyribonucleic acid is the genetic material in living organisms that is a double stranded…

Q: d). It's the temperature at which the mass of single stranded DNA equals the mass of double stranded…

A: Option d is NOT a correct description of the DNA melting temperature for a system in which we have…

Q: Consider the equation below. What are its consequences in the context of an electrophoresis…

A: Electrophoresis is a technique used in laboratories to separate DNA, RNA, and protein molecules…

Q: To study the chromatin structure of PIND, muclei from NIH3T3 cells and human growth hormone- treated…

A: DNAse 1 is an endonuclease with minimal sequence specificity. It can cleave both single-stranded and…

Q: How would the appearance of your DNA gel change if a 0.1% agarose gel slab was used? What about a 7%…

A: If 0.1% agarose gel slab was used then DNA cannot be seen clearly, it will break.Low…

Q: Would you expect the double helix in a short segment of DNA to be more stable in a storage solution…

A: DNA (deoxyribonucleic acid), is  a very complex molecule and is the primary carrier of genetic…

Q: Within living cells, many different proteins play importantfunctional roles by binding to DNA. Some…

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Q: oner micro mole of 48 nulceotide dna  was synthesised via solid phase .this sample contains salts…

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Q: Which of the following descriptions of regions A, B, and Care INCORRECT? A = minor groove, sugar…

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Q: Put the following pieces of DNA in order that they would appear reading from the top (nearest the…

A: First of all lets convert all of them into single unit 1kb = 1000bp So, 21000bp =21kb 10000bp = 10kb…

Q: pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI:…

A: Agarose gel electrophoresis is a method of gel electrophoresis that is used to separate a mixed…

Q: The chromosome of the bacterium (prokaryote) Escherichia coli has a size of approximately 4,000…

A: DNA stands for deoxyribonucleic acid. It carries genetic information useful for development and…

Q: You add 3.0 µg of plasmid DNA to a QIAprep spin miniprep purification column. You perform the…

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Q: please solve this with step-by-step calculations and explanations.

A: Here is handwritten answer

Q: 33.) The osmotlc pressure of the system is: (a) 1 atm (b) 0.016 atm (c) 6.5 X 105 atm (d) 22.59 atm…

A: BASIC INFORMATION  TRANSPORTATION  It is a process through which one substance move from one place…

Q: Calculate the electric fields and net force between a thymine and an adenine pair. = Calculate the…

A:

Q: You're purifying some plasmid DNA from a culture of bacteria and you want to know how pure it is.…

A: The purity of the resulting DNA must be assessed when plasmid DNA is purified from a bacterial…

Q: What is the melting temp. of the following double-stranded DNA fragment…

A: Melting temperature formula :  Tm = 4* (G+C) + 2 * (A+T)  Number of G+C = 6  Number of A+T = 20

Q: G CAT III || || || IIL| | III|| I | | II || | ||| || |L ||

A: Sanger sequencing, additionally called the “chain termination method”, maybe a technique for…

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A: Alkylating specialists substitute alkyl groups for hydrogen molecules on DNA, resulting in the…

Q: What is the role of GelRed® in Agarose gel electrophoresis of DNA fragments? Please select the…

A: Agarose gel electrophoresis is a method of gel electrophoresis used to separate a mixed population…

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Q: You have PGFPuv at 75ng / uL You need 1.15 ng total DNA in a final volume of 30 . Calculate how much…

A: pGFPuv encodes a green fluorescent protein (GFP) variant from Aequorea victoria that has been…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.