Sequencing of the following fragment of single-stranded DNA needs to be undertaken using the Sanger technique of dideoxynucleoside triphosphates. a) Draw the pattern of bands that would be seen after autoradiography. Remember this is not the strand you want. You need to make the complementary strand SC-C-A-C-C-T-A-G-C-CG-G3 b) You should draw a rectangle to represent the gel slab with four vertical columns headed, respectively, ddCTP, ddGTP, ddATP, and ddTTP. Draw in short horizontal lines to represent the bands as in the order they would appear.
Sequencing of the following fragment of single-stranded DNA needs to be undertaken using the Sanger technique of dideoxynucleoside triphosphates. a) Draw the pattern of bands that would be seen after autoradiography. Remember this is not the strand you want. You need to make the complementary strand SC-C-A-C-C-T-A-G-C-CG-G3 b) You should draw a rectangle to represent the gel slab with four vertical columns headed, respectively, ddCTP, ddGTP, ddATP, and ddTTP. Draw in short horizontal lines to represent the bands as in the order they would appear.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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