What can we most-accurately say about the polypeptide with the primary sequence KNEADING? a.It has two acidic R groups b.It has three basic R groups c. It has two nonpolar neutral R groups d. It contains three polar neutral R groups e. It contains a disulfide linkage

Q: nt #2 nt #3 nt # 4 3. In the following sequence, which of these nucleotides a. would have a free…

A: a) Nucleotides #1 and #5, because the 5’ end of each DNA strand has a triphosphate group.

Q: Consider the proteins in Figure 1.  Assume they are treated with chymotrypsin to cut them into…

A: Chymotrypsin:  It is a proteolytic enzyme that cuts the protein at a specific point. Proteolytic…

Q: A researcher is trying to purify a protein that needs to be in a reducing environment and wants to…

A: let's delve deeper into why DTT may interfere with the binding of the 6xHis-tagged protein to the…

Q: Consider an a-helix secondary structure. a. Propose a 12-residue amino acid sequence that(assuming…

A: In the study of protein structures, the alpha-helix is a prominent secondary structure characterized…

Q: As an example, consider albumin, a protein made of a single polypeptide weighing 66,000 daltons (66…

A: Gel electrophoresis if the technique of separation of molecules on the basis of their size and…

Q: 5’ - A T G    G C C    C A A    C T G    A C C - 3’ a. How many nucleotides are listed here b. How…

A: Transcription is the process by which the genetic information encoded in DNA is copied into a…

Q: Consider the equation below. What are its consequences in the context of an electrophoresis…

A: Electrophoresis is a technique used in laboratories to separate DNA, RNA, and protein molecules…

Q: 1. Explain why the primary structure sequence -Lys-Leu-Trp-Asp- may promote a-helix formation while…

A: The formation of α helix is occurred due to attachment of H of N of Cα of first (n) amino acid to…

Q: 28. a. Can a tRNA exist that has the anticodon sequence 5' IAA? If so, which amino acid would it…

A: The RNA present in prokaryote and eukaryotic cells, where DNA is main genetic material, is called…

Q: Why is Sanger sequencing referred to as the chain termination method? Select one: a. Sugars on ddNTP…

A: Sanger sequencing also known as the "chain termination method", is a method for determining the…

Q: Would you expect the double helix in a short segment of DNA to be more stable in a storage solution…

A: DNA (deoxyribonucleic acid), is  a very complex molecule and is the primary carrier of genetic…

Q: You are in the process of determining theamino acid sequence of a protein and must reconcile…

A: In the first trial, the protein has glycine at the amino terminal while asparagine at the carboxyl…

Q: 1. Consider the peptides P-A-I-G-E and M-A-T-T. a. Draw the structure of each peptide at a pH of 7.0…

A: Formation of the peptide bond neutralises any charge due to the alpha-amino group and alpha-carboxyl…

Q: What is the net charge of the peptide at pH 7 that is synthesized from this DNA sequence? 5'…

A: As we know that the DNA sequence exhibits codons, which helps in the formation of mRNA, and then…

Q: Consider the following pKa values: terminal amino group: 9.5; C = 8.2; Y = 10.1; K= 10.5; terminal…

A: Isoelectronic point is defined as the pH at which the amino acid does not move in an electric field.…

Q: Cyclic nucleotides (e.g., CGMP, CAMP) frequently make use of the same parts of the ribose ring;…

A: cGMP and cAMP are cyclic nucleotides where a cyclic bond is formed between sugar and phosphate…

Q: Which of the following could be the DNA template for the following protein primary structum…

A: The DNA is transcribed to produce the mRNA which gets translated into protein. Proteins are polymers…

Q: You're purifying some plasmid DNA from a culture of bacteria and you want to know how pure it is.…

A: The purity of the resulting DNA must be assessed when plasmid DNA is purified from a bacterial…

Q: Using the Figure below identify: What is the significance of hydrogen bonds in double helix of DN…

A: Deoxyribonucleic acid, or DNA, is a molecule that holds the information that allows an organism to…

Q: The following is diagram of a generalized tetranucleotide. Carbons exist at corners on the shapes…

A: DNA is the nucleic acid in the cell, which resides in the nucleus. It consists of the sequence of…

Q: A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When…

A: Size exclusion chromatography is a technique which separates molecules according to their size…

Q: 7. A protein has a molecular mass of 400 kDa when measured by gel filtration. When subjected to gel…

A: As we know that the sodium dodecyl (SDS) sulfate is considered as the denaturing agent, which helps…

Q: Which of the peptide sequences below best matches the hydropathy plot shown? 5 10 15 Residue Number…

A: Hydropathy plot is a quantitative analysis of the distribution of amino acids in a protein.…

Q: A Ramachandran Plot illustrates acceptable phi (φ) and psi (ψ) angles for amino acids in a…

A: Ramachandran plot is a representation of energetically favorable φ and ψ angles in polypeptides, for…

Q: 2.) It was Rosalind Franklin that helped Watson and Crick realize that the sugar-phosphate backbone…

A: Building blocks of DNA that had been known for many years yet the DNA structure was not known the…

Q: What is the function of sodium dodecyl sulfate (SDS) in SDS-PAGE? stabilizes the gel matrix,…

A: Electrophoresis means migration of charged particles under the influence of an electric field.…

Q: 40. What would you expect about the formation of an alpha-helix for a segment of a protein chain…

A: Two types of secondary structures are abundant in protein: alpha helix and beta sheets. The alpha…