Would you expect the double helix in a short segment of DNA to be more stable in a storage solution of sodium phosphat Duffer at pH 7.0 or in pure water? Why?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Would you expect the double helix in a short segment of DNA to be more stable in a storage solution of sodium phosphate
buffer at pH 7.0 or in pure water? Why?
pure water; because any cations in the storage solution would prevent complementary bases from forming
ionic interactions
sodium phosphate buffer at pH 7.0; because a neutral pH is required to maintain the covalent bonds between
complementary bases
O pure water; because the high dielectric constant of water is sufficient to stabilize the covalent bonds between
deoxyriboses in the DNA backbone
sodium phosphate buffer at pH 7.0; because the presence of water and sodium neutralizes the charge of phosphate
groups in the DNA backbone
Transcribed Image Text:Would you expect the double helix in a short segment of DNA to be more stable in a storage solution of sodium phosphate buffer at pH 7.0 or in pure water? Why? pure water; because any cations in the storage solution would prevent complementary bases from forming ionic interactions sodium phosphate buffer at pH 7.0; because a neutral pH is required to maintain the covalent bonds between complementary bases O pure water; because the high dielectric constant of water is sufficient to stabilize the covalent bonds between deoxyriboses in the DNA backbone sodium phosphate buffer at pH 7.0; because the presence of water and sodium neutralizes the charge of phosphate groups in the DNA backbone
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