What would the net charge be of a polypeptide with the sequence M-D-R-N-Q-K at pH 8? hint ionizable groups include N-terminus (pKa = 9.0), D (pKa = 3.9), R (pKa = 12.5), K (pKa = %3D %3D 10.5) O+2 O +1 O-1

What would the net charge be of a polypeptide with the sequence M-D-R-N-Q-K at pH 8? hint ionizable groups include N-terminus (pKa = 9.0), D (pKa = 3.9), R (pKa = 12.5), K (pKa = %3D %3D 10.5) O+2 O +1 O-1

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.

Question

What would the net charge be of a polypeptide with the
sequence M-D-R-N-Q-K at pH 8? hint ionizable groups include
N-terminus (pKa = 9.0), D (pKa = 3.9), R (pKa = 12.5), K (pKa =
%3D
%3D
%3D
%3D
10.5)
O +2
O+1
O-1
00

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