2.Calculate the overall charge (pH 7) on the following three polypeptides and answer thequestions below. Assume the following pKa values: N-terminal –NH3®, 7.0; all -COOH groups, 4.0%3;Arg, 12.5; Cys, 8.4; His, 6.0; Lys, 10.0; Tyr, 10.0.(A)Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg-Arg-Pro-Val-Lys-Val-Tyr-Pro-Asp-Ala-Gly- Glu--Asp-Gln- Ser-Ala-Glu-Ala-Phe-Pro-Leu-Arg-Glu-Phe(B)Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Ala-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu-Val-Tyr-Pro-Asp-Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu-Phe-Cys-Ser-Tyr-Ser-Met-Glu-His-Phe-Asp-Trp-Gly-Asp-Pro-Val-Gly-Pro-Asp-Ala-Gly-Asp-Gln-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu-Val-Tyr-Pro-Asp-Ala(C)Gly-Ser-Val-Arg-Asp-Pro-Val–Lys–Glu–Val–Tyr-Pro–Asp- Lys-Ala-Gly-Arg–Glu–Ser-Arg-Ala(h) Which of the peptides would migrate the closest to the cathode in isoelectric focusing?(i) Which of the three peptides would migrate the furthest away from the cathode in isoelectric focus-ing?(j) Which of the following three peptides could potentially migrate at a different rate during gel filtra-tion in the absence or in the presence of dithiothreitol (DTT), a compound used for reducing disulfidebridges?

Proteins or peptides are composed of twenty standard amino acids that differ from each other on the…

Answered: 2. Calculate the overall charge (pH 7)…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL with water (i.e. 0.1 mL of the solution was added to 3.9 mL of water). 2 mL of this solution was then mixed with 18 mL of water. What is the concentration of the diluted protein solution? Space to show your workings:
In a 0.1000 M acetic acid solution at 25 degrees celsius , the acid ionizes to the extent of about 1.34 %. Since each molecule of acetic acid which ionizes produces 1 H+ ion and 1 C2H3O2- ion, the concentration in the solution are: HC2H3O2 < -----------> H+ + C2H3O2-
2. . questions below. Assume the following pKa values: N-terminal –NH3®, 7.0; all -COOH groups, 4.0; Arg, 12.5; Cys, 8.4; His, 6.0; Lys, 10.0; Tyr, 10.0. Calculate the overall charge (pH 7) on the following three polypeptides and answer the (A) Ser-Tyr-Ser-Met-Glu–His–Phe–Arg-Trp-Gly-Lys-Pro–Val-Gly-Lys-Lys-Arg-Arg-Pro-Val-Lys–Val-Tyr-Pro-Asp-Ala -Gly- Glu--Asp-Gln- Ser-Ala-Glu-Ala-Phe-Pro-Leu-Arg-Glu-Phe (B) Ser-Tyr-Ser-Met-Glu–His–Phe-Arg-Trp-Gly-Ala-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu–Val-Tyr-Pro-Asp- Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu–Phe-Cys-Ser-Tyr-Ser-Met–Glu–His-Phe-Asp-Trp-Gly- Asp-Pro-Val-Gly-Pro-Asp-Ala-Gly-Asp-Gln-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu-Val-Tyr-Pro-Asp-Ala (C) Gly-Ser-Val-Arg-Asp-Pro-Val-Lys-Glu-Val-Tyr-Pro-Asp- Lys-Ala-Gly-Arg-Glu-Ser-Arg-Ala (g) Which of the three peptides would migrate the furthest away from the anode in isoelectric fo- cusing (h) Which of the peptides would migrate the closest to the cathode in isoelectric focusing?…
2. You made a pH=2.5 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in total concentration of glycine. (pka1=2.34 and pka2=9.69) a) What 2 glycine species are present in the buffer solution at this pH? (draw their structures) b) What is the concentration ratio of these 2 species? c) How many moles of each species, A- and HA, is there in 500 ml of the buffer solution. d) If 25 ml of 0.82 M HCI is added to 500 ml of this buffer solution. What reaction is taking place? What is the final pH of the solution? Is the solution still a buffer? e) Draw the titration curve, pH versus equivalents of OH-, for glycine and determine the pl.
2
1. Isoelectric point of polypeptide: (1) is pH at which the o-carboxyl and o-amino groups are uncharged. (2) is pH at which the net charge on the molecules in solution is 0. (3) is pH at which the charge of the o-carboxyl group is higher than the charge of the o- amino group. А. (1) only В. (2) only С. (3) only D. (1) and (3) E. (2) and(3)
1. What will happen if there is a change in the configuration of a protein molecule? Provide an example. 2. Give the IUPAC name for Ala-Gly-Leu
1. What is the possible identity of the AA? a. Aspartic acid b. Lysine c. Alanine d. Proline 2. What is the isoelectric point of AA? 3. What is the pKa responding to the dissociation of the alpha-carboxylic group?
1. Draw the tetrapeptide Met-Ala-Thr-Thr at a ph of 7? 2. Draw the tetrapeptide Met-Ala-Thr-Thr at a ph of 12?
1. Draw the structures of the 20 biologically active AA. Under each structure, write the name, one-letter code, and three-letter code followed by the AA classification based on polarity.
5. Refer to Model 2 for information relevant to this question. A sample of the peptide Lys-Glu-Ser has a net charge of zero between what two pH values? What is the pl of Lys-Glu-Ser? Model 2: The pl of a peptide is determined by examining the ionizable groups. The protonated and unprotonated forms of each ionizable group are in equilibrium. Consider the peptide Lys-Glu-Ser shown below at pH 7.2. The complete structure is on on the left and a stylized structure with just the ionizable groups is on the right. While the N-terminal is depicted as protonated, a sample of Lys-Glu-Ser is composed of a population of molecules and within that population some molecules contain a non-protonated N-terminal group at pH 7.2. may H3N* COO- H3N-CH-CIN-CH C N-CH C- H. pK 3.5 OH H. pK= 8.5 COO- CH2 CH2 CH2 NH, pK 10.0 pK 4.2 CH2 CH2 OH CH2 c=0
1. Examine the synthetic glycoprotein, Molecule A, shown below: Protein- OH OH OH OH OH ÓH OH Molecule A OH Draw the structures of the products obtained when Molecule A reacts under the following conditions. Justify your answer. If no reaction occurs, explain why. The monosaccharide rings that have not reacted may be represented by an “R" group. Mechanisms are not required. (а) Ammonium Carbonate, 5 days. (b) Excess Sodium Periodate. (c) 2,3-dinitrophenyl hydrazine under reductive amination conditions. NO2 O2N. „NHNH2 2,3-dinitrophenyl hydrazine

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