In a Sanger DNA sequencing reaction (dideoxy method) you use the primer 5′-GCATATA-3′ to sequence a DNA strand that starts with the sequence 3′-CGTATATCCCTACGTTGG-5′ (consider the strand about 100-nucleotide long). You try your sequencing reaction three times, but every time you make a mistake, as indicated below. What would be the outcome in each case? Justify your answers. (a) You forgot to add dCTP (b) You forgot to add ddCTP (c) Your primer is complementary to two regions in the DNA to be sequenced.

Q: describe what would occur if cells were in mitosis more than they were in interphase?

A: Mitosis is a cell division which gives rise to genetically identical cells. The cell division…

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A: Restriction enzymes are the specific enzymes that recognize and cut at specific sites on nucleotide…

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Q: 1f) If you took more of the same randomly generated 1000 bp fragments of binturong DNA (the same…

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A: DNA methylation is a chemical change that frequently takes place in the setting of CpG dinucleotides…

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A: It is a Polymerase Chain Reaction, which is a mechanism for multiplying a specific piece of DNA…

Q: You were going to sequence a rice DNA fragment whose sequence was only know at one end, as shown…

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Q: List essential components required for the DNA sequencing reaction based on the Sanger method.…

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Q: What was the function of each solution or step in the DNA purification protocol? The following…

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A: It is related to molecular biology in which we use restriction enzymes an important tool for

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Q: Can you please help with 1d please  picture with 1 graph is for question 1a) picture with 4 graphs…

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Q: 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp…

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Q: How often, on average, will the endonuclease AluI, which cleaves the sequence 5’ AGCT 3’, cut normal…

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A: Genomic Libraries are a set of collections of genomic DNA data particular to a species/organism. The…

Q: The sequence of the 15 bp fragment from the previous problem is repeated below: 5' TCTGAATTCCGTAGA…

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Q: You have a very concentrated sample of DNA which you need to dilute for sequencing 1:6000 with a…

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Q: Examine the sequence for the DNA fragment below. Your job is to design primers for PCR that would be…

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.