In a Sanger DNA sequencing reaction (dideoxy method) you use the primer 5′-GCATATA-3′ to sequence a DNA strand that starts with the sequence 3′-CGTATATCCCTACGTTGG-5′ (consider the strand about 100-nucleotide long). You try your sequencing reaction three times, but every time you make a mistake, as indicated below. What would be the outcome in each case? Justify your answers. (a) You forgot to add dCTP (b) You forgot to add ddCTP (c) Your primer is complementary to two regions in the DNA to be sequenced.

Q: describe what would occur if cells were in mitosis more than they were in interphase?

A: Mitosis is a cell division which gives rise to genetically identical cells. The cell division…

Q: Which RE’s from table below have a 5’ overhang? Which ones have a 3’ Overhang?   AccI GT^CGAC…

A: Restriction enzymes are the specific enzymes that recognize and cut at specific sites on nucleotide…

Q: A) If one of the primers has a point mutation distinct from the template what is the percentage of…

A: PCR or polymerase chain reaction is a technique which is used in amplification of DNA or produce…

Q: scientist is interested in the amplification of a specific DNA fragment in a large plasmid of about…

A: The specific DNA sequence can be amplified by using primers in the polymerase chain reaction or PCR.…

Q: Your company has decided to develop a PCR test that will be used to determine Huntington's disease.…

A: The polymerase chain reaction (PCR), is one of the most useful molecular biology techniques used to…

Q: 1f) If you took more of the same randomly generated 1000 bp fragments of binturong DNA (the same…

A: Deoxyribonucleic acid  is a particle made out of two polynucleotide chains that loop around one…

Q: A researcher is interested in using the in vitro technique of bisulfite conversion to confirm the…

A: DNA methylation is a chemical change that frequently takes place in the setting of CpG dinucleotides…

Q: Your graduate advisor asks you to amplify the following sequence of DNA by PCR:…

A: The primer should be complementary to the region of template to be amplified.

Q: Design PCR primers for the DNA sequence given below and explain your choice. As part of your answer,…

A: It is a Polymerase Chain Reaction, which is a mechanism for multiplying a specific piece of DNA…

Q: You were going to sequence a rice DNA fragment whose sequence was only know at one end, as shown…

A: A primer is a brief sequence of single-stranded DNA that is utilized in the PCR method of polymerase…

Q: A plot showing the % of denaturation as a function of temperature for a melting point of a DNA…

A: DNA is a double stranded helically wound macromolecule. Each strand is made up of a sequence of…

Q: A virus with a circular double-stranded DNA chromosome contains approximately 10,000 bp. You want to…

A: To construct a map of the viral chromosome based on the cleavage sites of the restriction…

Q: List essential components required for the DNA sequencing reaction based on the Sanger method.…

A: DNA sequencing is the process of determining the sequence of nucleotides  in a piece of DNA. In…

Q: A facility says they need 15 μL of a 40 ng/μL solution of plasmid DNA for sequencing. The typical…

A: Appropriate amount of DNA is one the key elements in gene sequencing. Amount of DNA for sequencing…

Q: What was the function of each solution or step in the DNA purification protocol? The following…

A: Deoxyribonucleic acid extraction is the isolation and purification of DNA from sample using a…

Q: How would you solve this question? I am confused

A: The objective of this question is to estimate the size of an unknown DNA fragment using a standard…

Q: When circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed…

A: It is related to molecular biology in which we use restriction enzymes an important tool for

Q: A team of scientist is interested in the amplification of a specific DNA fragment in a large plasmid…

A: The specific DNA sequence can be amplified by using primers in the polymerase chain reaction or PCR.…

Q: Your PhD thesis advisor has given you the task of preparing a human genomic DNA library.  3a. How…

A: The length of the human genome is approximately 3.2 billion bases with genes of 20000-25000 protein…

Q: Can you please help with 1d please  picture with 1 graph is for question 1a) picture with 4 graphs…

A: Binturong or the bearcat is found in South and Southeast Asia. It is declared as vulnerable,…

Q: 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via a…

Q: The restriction endonuclease NotI recognizes the octanucleotide sequence GCGGCCGC. Calculate the…

A: Restriction endonucleases are enzymes that cut DNA sequences at a specific site known as a…

Q: How often, on average, will the endonuclease AluI, which cleaves the sequence 5’ AGCT 3’, cut normal…

A: Enzyme restriction, also referred to as endonuclease restriction, is a protein formed by bacteria…

Q: Ailee is interested to determine the nucleotide sequence of her bacterial heat shock gene. Hence,…

A: In response to temperature changes, the heat-shock response involves the induction of many proteins…

Q: Which one of the following options would be a good way to identify the location of the poly A tail…

A: Pre mRNA undergoes several post-transcriptional modifications. One of these modifications is…

Q: a)Dr. Thisisaneasyexam decides to amplify a gene from a plasmid using PCR. She starts out with 6.6 x…

A: Sure, let me provide a more detailed explanation for the calculations.a) Calculating the number of…

Q: Assume that a DNA sequencing reaction is carried out, except that the four different…

A: Ans: DNA sequencing: It is the process of knowing the sequence of the DNA. The DNA sequencing is of…

Q: Human genomic libraries used for DNA sequencing are often made from fragments obtained by cleaving…

A: Genomic Libraries are a set of collections of genomic DNA data particular to a species/organism. The…

Q: Examine the DNA sequence shown below. You have been tasked with designing Primers for PCR…

A: Polymerase chain reaction (PCR) is a method used to  make millions of copies of a specific DNA…

Q: The sequence of the 15 bp fragment from the previous problem is repeated below: 5' TCTGAATTCCGTAGA…

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Q: A 1% by weight agarose gel is typically used in agarose gel electrophoresis. If you used a 2%…

A: During agarose gel electrophoresis, an agarose solution is prepared, it is cooled to prepare a gel,…

Q: You have a very concentrated sample of DNA which you need to dilute for sequencing 1:6000 with a…

A: Dilution is defined as the ratio of volume from stock to the final volume.  Dilution= Stock volume /…

Q: Examine the sequence for the DNA fragment below. Your job is to design primers for PCR that would be…

A: A primer may be a brief single-stranded nucleic acid utilized by all living living beings within the…

Q: Which of the following will lead to DNA cutting more frequently? Choose all that apply.

A: The restriction enzymes are enzymes that cut the DNA at specific nucleotide sequences called the…

Q: You have determined that the newly synthesized strand of DNA in your sequencing reaction has the…

A: Template Strand will be: 3' TGATGGCTCA5'

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.