**Sanger Sequencing Autoradiogram Analysis**In this exercise, a 13-nucleotide section of an autoradiogram from a Sanger sequencing experiment is provided. The goal is to interpret the band pattern and determine the sequence of both the complementary strand generated during the experiment and the original template strand being analyzed.**Band Patterns and ddNTP Incorporation:**The image displays a sequencing gel with four lanes, each labeled at the top with a specific dideoxynucleotide triphosphate (ddNTP): ddATP, ddGTP, ddCTP, and ddTTP. Each lane corresponds to a specific nucleotide terminator used in the sequencing reaction.**Description of the Gel Diagram:**- **Lane ddATP:** Contains bands at various positions.- **Lane ddGTP:** Contains bands at various positions.- **Lane ddCTP:** Contains bands at various positions.- **Lane ddTTP:** Contains bands at various positions.The bands in each lane represent DNA fragments that terminated at that specific ddNTP. Reading from the bottom (5' end) to the top (3' end) of the gel provides the sequence of the complementary strand in the 5’ to 3’ direction.**Interpreting the Sequences:**1. **Complementary Strand Sequence (from Gel):** - Read the sequence in the 5’ to 3’ direction from the bottom to the top of the gel by locating the position of each band and noting the corresponding ddNTP.2. **Template Strand Sequence:** - Once the complementary strand sequence is determined, calculate the template strand by converting each base to its complement (A↔T, C↔G).Be sure when completing the exercise to clearly indicate the 5’ and 3’ ends of both strands. This understanding is crucial for determining the correct DNA sequence of the original sample.

The DNA is made up of nucleotides that contain nitrogenous bases, pentose sugar and phosphate group. DNA contain two complementary strands that are remain bounded together by hydrogen bonds.In Sanger sequencing we use ddNTPs and dNTPs. The ddNTPs (dideoxyribonucleotide triphosphate) causes chain termination when they are incorporated in the DNA. The process is based on the replication mechanism. The data shows the fragments of newly synthesized DNA. The DNA synthesis occurs in 5' to 3' direction of the new strand. The smallest fragments move further and largest fragments move least in the gel bed. The sequence of the complementary strand that is generated during the experiment is 5'-TACAGTTTCAGGA-3' The sequence of the template strand that is being analysed in this experiment is 3'-ATGTCAAAGTCCT-3'.